Addgene

We narrowed to 6,032 results for: WUS;

Showing: 1411 - 1440 of 6032 results

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pET21a-hAIM2-His6

Plasmid

#51532

Purpose

Express His6-tagged hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pUGI-NLS

Plasmid

#101091

Purpose

Expresses UGI in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDB4697

Plasmid

#171125

Purpose

A plasmid containing the ura4 genomic region,which flanked by a NotI site at the both ends. It served as the control plasmid or backbone for the ura4 integrated plasmid construction.

Depositor

Li-Lin Du

Article

Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

Insert

ura4

Use

Other

Tags

Expression

Mutation

non applicable

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP720

Plasmid

#197241

Purpose

Expresses the fusion protein of Tubulin-miRFP720 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-miRFP720

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP680-HO1

Plasmid

#197253

Purpose

Expresses the protein of miRFP680 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:(MCS)-S11

Plasmid

#108179

Purpose

Binary vectors used for the inducible expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

UBQ10:sXVE:

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-BRIC

Plasmid

#172341

Purpose

AAV transfer plasmid for BRIC (Bioluminescent Red Indicator for Calcium)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BRIC

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0mut

Plasmid

#153290

Purpose

Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in astrocytes

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)

Use

AAV

Tags

Expression

Mutation

Promoter

GfaABC1D

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTomatoSlicer

Plasmid

#221085

Purpose

Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP720-HO1

Plasmid

#197257

Purpose

Expresses the protein of miRFP720 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP720

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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CAG.CenpB(FL)-GFP-Halo

Plasmid

#176205

Purpose

Centromeric anchor for TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

CenpB-GFP-Halo

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCB301TOR-CRISPR

Plasmid

#164797

Purpose

For gene editing in oomycetes

Depositor

Miaoying Tian

Article

Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

Inserts

PsNLS-hSpCas9
sgRNA expression cassette

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

HAM34 and RPL41

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDsRed-monomer-hAIM2

Plasmid

#51544

Purpose

Express DsRed monomer-tagged hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

DsRed monomer

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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ABE8r-V106W

Plasmid

#208295

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-V106W-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDN-LexAOx4-mNeonGreen

Plasmid

#192604

Purpose

Vector for inducible mNeonGreen expression with 4x LexA operator (LexAO)

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

mNeonGreen

Use

Unspecified

Tags

Expression

Mutation

Promoter

Minimal promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-FKBP12-C1

Plasmid

#67900

Purpose

Expresses a fusion of mCherry with FKBP12 in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-FKBP12

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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ABE8r-R153del

Plasmid

#208296

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-R153del-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-jRCaMP1a

Plasmid

#113936

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jRCaMP1a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTNSQCas

Plasmid

#219434

Purpose

CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

TnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-LoxP

Plasmid

#219436

Purpose

CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

two transposon ends, LoxP-terminator-LoxP gene cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-mAIM2

Plasmid

#51540

Purpose

Express Flag-tagged mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCytERM-BDFP1.6-N1

Plasmid

#197179

Purpose

Expresses the fusion protein of CytERM-BDFP1.6 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-BDFP1.6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-NLS-LSSmKate2

Plasmid

#34586

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS-LSS-mKate2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Promoter

Available since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pBAD-KIRIN1-GR

Plasmid

#107417

Purpose

Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1-GR

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1-GR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pJWV25

Plasmid

#61045

Purpose

To construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaA

Depositor

Jan-Willem Veening

Article

Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

Type

Empty backbone

Use

Synthetic Biology

Tags

GFP+

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-mad

Plasmid

#188998

Purpose

Express wildtype madanin-1

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

wildtype madanin-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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MPZ-intron1 pGL3-TATA

Plasmid

#21630

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

myelin protein zero intron1 (Mpz Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pBAD-R-GECO1.2

Plasmid

#113937

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1.2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP680

Plasmid

#197239

Purpose

Expresses the fusion protein of Tubulin-miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-miRFP680

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRVdG-4mCherry

Plasmid

#52488

Purpose

Expresses mCherry

Depositor

Sebastian Seung

Article

Weible et al J Neurosci. 2010 Dec 8;30(49):16509-13.

Insert

mCherry

Use

Rabies virus

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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