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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,743 results for: sequence

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Showing: 1411 - 1440 of 13743 results

  1. CROPseq-Puro-24xMS2

    Plasmid
    #212629
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    212629-plasmid-map-sequence-id-420222

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  4. pJC.15A.sgRNA.TA

    Plasmid
    #232483
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.
    Depositor
    James Collins
    Article
    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0
    Inserts
    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    P3 constitutive promoter and native promoter
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232483-plasmid-map-sequence-id-466415

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  18. pJES103

    Plasmid
    #135410
    Purpose
    E. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporter
    Depositor
    Miriam Braunstein
    Article
    McCann et al Microbiology. 2007 Oct;153(Pt 10):3350-9
    Insert
    blaTEM-1
    Expression
    Bacterial
    Mutation
    E. coli 'blaTEM-1 with M. tuberculosis mpt63…
    Promoter
    hsp60
    Available Since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135410-plasmid-map-sequence-id-270631

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  19. pYPQ262m3

    Plasmid
    #149556
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, R221K, N394K, Protospacer interacting domai…
    Available Since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149556-plasmid-map-sequence-id-306141

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  27. pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H

    Plasmid
    #108375
    Purpose
    FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    108375-plasmid-map-sequence-id-209429

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Showing: 1411 - 1440 of 13743 results