We narrowed to 3,314 results for: GCT

1. pCY5.1nh

   Plasmid

   #160299

   Purpose

   Yeast CRISPR plasmid targeting the natMX and hphMX cassettes

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
   URA3MX selection cassette
   sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDL649

   Plasmid

   #231164

   Purpose

   T-DNA encoding TRV2 with mobile gRNA targeting SlHAIRLESS

   Depositor

   Daniel Voytas

   Article

   Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

   Insert

   mobile gRNA targeting SlHAIRLESS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PEBV sub-genomic

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDL518

   Plasmid

   #231160

   Purpose

   T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

   Depositor

   Daniel Voytas

   Article

   Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

   Insert

   mobile gRNA targeting SlPDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PEBV sub-genomic

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEE771

   Plasmid

   #231159

   Purpose

   T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

   Depositor

   Daniel Voytas

   Article

   Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

   Insert

   mobile gRNA targeting SlPDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PEBV sub-genomic

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEE485

   Plasmid

   #231158

   Purpose

   T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

   Depositor

   Daniel Voytas

   Article

   Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

   Insert

   mobile gRNA targeting SlPDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PEBV sub-genomic

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. gRNA giantin/GOLGB1

   Plasmid

   #222315

   Purpose

   CRISPR/Cas9 close to the ATG of giantin/GOLGB1 gene.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

   Insert

   gRNA targeting GOLB1 (GOLGB1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDL655

   Plasmid

   #231165

   Purpose

   T-DNA encoding gRNA targeting SlPDS

   Depositor

   Daniel Voytas

   Article

   Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

   Insert

   gRNA targeting SlPDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDL562

   Plasmid

   #231162

   Purpose

   T-DNA encoding TRV2 with mobile gRNA targeting SlCHL1

   Depositor

   Daniel Voytas

   Article

   Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

   Insert

   mobile gRNA targeting SlCHL1
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PEBV sub-genomic

   Available since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEE834

   Plasmid

   #231168

   Purpose

   T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

   Depositor

   Daniel Voytas

   Article

   Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

   Insert

   mobile gRNA targeting SlPDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PEBV sub-genomic

   Available since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RhoA gRNA #3

    Plasmid

    #198720

    Purpose

    Cas9-mediated knockout of RhoA in mammalian cells #3

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    RhoA (Rhoa Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-nucGFP11-3xPax7gRNA

    Plasmid

    #224570

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-3xGFP11-mem-3xPax7gRNA

    Plasmid

    #224571

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-hU6-2O-crEGFP_EF1a-BFP

    Plasmid

    #224784

    Purpose

    EGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crEGFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC-hU6-CrEGFP_EF1a-BFP

    Plasmid

    #224786

    Purpose

    EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYAMTr2GCsgSc-SeLEU2

    Plasmid

    #224871

    Purpose

    Disruption of LEU2 genes in the Saccharomyces sense strict group species

    Depositor

    Katsunori Suzuki

    Article

    Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

    Insert

    pYAMTr2GC having the guide sequence Sc-SeLEU2 (LEU2, DI49_0736 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSuperior.retro.neo GFP shDNM1L

    Plasmid

    #220356

    Purpose

    Retroviral expression vector for an shDNM1L

    Depositor

    David Kashatus

    Article

    Sweatt et al Mol Syst Biol. 2024 Sep 27. doi: 10.1038

    Insert

    shDNM1L (DNM1L Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. LT3GEPIR-WT1shRNA

    Plasmid

    #220560

    Purpose

    To inducibly knockdown WT1 or EWSR1::WT1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

    Insert

    WT1 shRNA (WT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDB_131

    Plasmid

    #216095

    Purpose

    Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD97, CD4, CD26, CD274 guides
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCF827-sgCIDE-2_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Plasmid

    #211697

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgCIDE-2 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCF826-sgCIDE-2_U6-sgRNA-EFS-Cas9-P2A-mCherry2

    Plasmid

    #211691

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-mCherry2

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgCIDE-2 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LENTICRISPR-SNRPA_sgRNA1

    Plasmid

    #201622

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    SNRPA (SNRPA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Lenti-guide-puro mRab12-1

    Plasmid

    #198475

    Purpose

    lentiviral stable expression of mRab12 gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mouse Rab12 (Rab12 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLKO.1-Teton-puro-shPP1C #1

    Plasmid

    #198762

    Purpose

    conditional knockdown of PP1C

    Depositor

    Hyonchol Jang

    Article

    Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

    Insert

    shPP1C #1 (PPP1CC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pXPR_003 sgGPAA1 guide 2

    Plasmid

    #193609

    Purpose

    GPAA1 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgGPAA1 guide 2 (GPAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pXPR_003 sgAP2M1 guide 1

    Plasmid

    #193600

    Purpose

    AP2M1 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgAP2M1 guide 1 (AP2M1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pXPR_003 sgFADD guide 1

    Plasmid

    #193589

    Purpose

    FADD knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgFADD guide 1 (FADD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXPR_003 sgFADD guide 2

    Plasmid

    #193590

    Purpose

    FADD knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgFADD guide 2 (FADD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PB-SRT-Puro_BC13

    Plasmid

    #193155

    Purpose

    Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

    Depositor

    Rob Mitra

    Article

    Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

    Insert

    Barcoded piggyBac Puro SRT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Barcode: GCTA

    Promoter

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-Sa-gRNA-puro

    Plasmid

    #191652

    Purpose

    Lentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    S. aureus gRNAscr
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSCL.179

    Plasmid

    #185000

    Purpose

    Express -Eco1 HEK4 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    HEK4 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. E42_gRunx3_gRNA3_dTet_mTurquoise2

    Plasmid

    #189806

    Purpose

    Retroviral delivery of guide RNA against mouse Runx3

    Depositor

    Ellen Rothenberg

    Article

    Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    gRunx3_gRNA3 (Runx3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti-v2-ALDH1A3gRNA2

    Plasmid

    #189747

    Purpose

    The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    Cas9, ALDH1A3 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSLiP-G1G2

    Plasmid

    #188965

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Tol2-LyzC-PBD-GFP-U6ac-rac2 guides

    Plasmid

    #168251

    Purpose

    "label active Rac under Rac2 neutrophil-specific knockout background"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    PBD GFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    LyzC

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

    Plasmid

    #168254

    Purpose

    "label stable F-actin under Rac2 neutrophil-specific knockout background"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Utrophin-GFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    LyzC

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mU6-sgCebpa_v1-hu6-sgCebpb_v1

    Plasmid

    #177251

    Purpose

    Expresses Cebpa_v1 (mU6), Cebpb_v1 (hU6) gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgCebpa_v1/sgCebpb_v1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6/hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mU6-sgCebpb_v1-hu6-sgCebpd_v1

    Plasmid

    #177255

    Purpose

    Expresses Cebpb_v1 (mU6), Cebpd_v1 (hU6) gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgCebpb_v1/sgCebpd_v1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6/hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. hU6-sgNT1 (opti)

    Plasmid

    #177237

    Purpose

    Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses non-targeting gRNA

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgNT1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. hU6-sgNeo1 (opti)

    Plasmid

    #177240

    Purpose

    Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses neomycin gRNA

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgNeo1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. hu6-sgCebpa_v1 (opti)

    Plasmid

    #177245

    Purpose

    Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpa gRNA

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgCebpa_1st
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge