Skip to main content
Addgene

We narrowed to 8,867 results for: BLI;

Showing: 2351 - 2400 of 8867 results
  1. pGR481

    Plasmid
    #213783
    Purpose
    Integrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutations
    Depositor
    Insert
    ura3*
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pJK645

    Plasmid
    #71700
    Purpose
    Produces E. coli thioredoxin (EcTrxA)
    Depositor
    Insert
    thioredoxin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. Rich-pT7

    Plasmid
    #108445
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    pT7
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pGloSensor -40F cGMP Vector

    Plasmid
    #236812
    Purpose
    Express -40F cGMP in biosensor assay
    Has Service
    DNA
    Insert
    -40F
    Use
    Luciferase
    Tags
    Expression
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. pGloSensor -42F cGMP Vector

    Plasmid
    #236806
    Purpose
    Express -42F cGMP in biosensor assay
    Has Service
    DNA
    Insert
    -42F
    Use
    Luciferase
    Tags
    Expression
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pAG GST EGFP

    Plasmid
    #236736
    Purpose
    Bacterial expression plasmid containing GST-tagged EGFP
    Insert
    EGFP
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    April 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. pWALIUM10-GW-TurboID-V5

    Plasmid
    #196283
    Purpose
    Gateway destination vector to express C-terminal TurboID fusion under UAS promoter, for making transgenic flies
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pBIR-GFP

    Plasmid
    #49807
    Purpose
    In vivo GFP based system to measure break induced replication (BIR) repair efficiency. expression of I-SceI generates a double strand break that when repaired by BIR mechanism will restore GFP.
    Depositor
    Insert
    GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    beta-actin
    Available since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pTYB1-T4uvsX

    Plasmid
    #228213
    Purpose
    Expression in BL21(DE3) of T4 recombinase
    Depositor
    Insert
    uvsX (uvsX Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. PB CAG-iCre-ERT2

    Plasmid
    #137858
    Purpose
    CAG-iCre-ERT2 piggyBac transposon
    Depositor
    Insert
    iCre-ERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pMCMV3

    Plasmid
    #85711
    Purpose
    Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MIEPmouse CMV MIEP
    Available since
    April 15, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. LentiCRISPRv2 RFP670

    Plasmid
    #187646
    Purpose
    lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site
    Depositor
    Insert
    RFP 670
    Use
    Lentiviral
    Tags
    RFP670 and sgRNA cloning site
    Expression
    Mutation
    Promoter
    EFS (P2A)
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pRRlsinPGK_CREGFP_WPRE

    Plasmid
    #91789
    Purpose
    Constitutive expression of creGFP fusion using PGK promoter in a lentiviral backbone.
    Insert
    Cre-GFP fusion protein
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  14. pGMC00009 (aka pRC0494)

    Plasmid
    #195294
    Purpose
    Lenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFP
    Depositor
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  15. pLV Nup62-V5

    Plasmid
    #175116
    Purpose
    Lentiviral expression of mouse Nup62-V5
    Depositor
    Insert
    Nup62 (Nup62 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pSpot-Tag-GFP-GPI

    Plasmid
    #117061
    Purpose
    For mammalian expression of GPI-anchored GFP with N-terminal Spot-Tag
    Depositor
    Insert
    GPI-anchored GFP
    Use
    Tags
    GPI-anchor and Spot-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. pChillyBoys_gen1

    Plasmid
    #228495
    Purpose
    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
    Depositor
    Inserts
    groEL
    groES
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  18. CROP-seq-opti-dsRed

    Plasmid
    #201999
    Purpose
    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  19. p3E_he1a:eYFP

    Plasmid
    #113879
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    eYFP
    Expression
    Mutation
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  20. pLV V5-Nesprin-3

    Plasmid
    #175136
    Purpose
    Lentiviral expression of V5-tagged mouse Syne3
    Depositor
    Insert
    Syne3 (Syne3 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  21. pGEM4Z-OMA1

    Plasmid
    #61718
    Purpose
    human Oma1 protease in the E.coli/in vitro translation vector
    Depositor
    Insert
    OMA1 (OMA1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
  22. pAAV.synP.DIO.EGFP.WPRE.hGH

    Plasmid
    #100043
    Purpose
    An AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).
    Depositor
    Has Service
    AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin-1
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
  23. XLone-PE2

    Plasmid
    #136463
    Purpose
    Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment
    Depositor
    Insert
    PE2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GS
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  24. Clover2-N1

    Plasmid
    #54537
    Purpose
    Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Clover2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  25. p3E_he1a:mCherry

    Plasmid
    #113881
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
  26. pUC119-MCS

    Plasmid
    #58807
    Purpose
    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
  27. pCer-C3-Plk1

    Plasmid
    #68132
    Purpose
    Expression of Cerulean-tagged Plk1 in mammalian cells
    Depositor
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
  28. pcDNA3.1-HA-Bax

    Plasmid
    #191944
    Purpose
    Expression of HA-Bax.
    Depositor
    Insert
    HA-Bax (Bax Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
  29. pBEST-p15a-pBAD-UTR1-TetR-T500

    Plasmid
    #45787
    Depositor
    Insert
    Tetracycline repressor
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 16, 2013
    Availability
    Academic Institutions and Nonprofits only
  30. pmR-mCherry miR-31

    Plasmid
    #84916
    Purpose
    mammalian expression of miR-31
    Depositor
    Insert
    miR-31 (Mir31 Mouse)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
  31. pExp-TF

    Plasmid
    #112575
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    8xHis-TriggerFactor and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  32. pShuttle-CMV-F2A-T2A

    Plasmid
    #62622
    Purpose
    AdEasy Shuttle vector modified for multicistronic expression. Contains T2A and F2A
    Depositor
    Insert
    MCS with F2A and T2A
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  33. pAG Retro Puro Empty

    Plasmid
    #234458
    Purpose
    Retroviral vector for cloning and expressing your gene of interest. Puromycin selection.
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  34. pLenti_PGK_Prkaa1

    Plasmid
    #204356
    Purpose
    Lentiviral plasmid, Expresses Prkaa1 under the control of the PGK promoter
    Insert
    AMP-activated protein kinase alpha 1 (Prkaa1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
  35. pWALIUM10-V5-TurboID-GW

    Plasmid
    #196285
    Purpose
    Gateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic flies
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  36. pLIX403-ccdB-EBFP2

    Plasmid
    #158562
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  37. pCMVlac-dirTopo-P2A-aGFP

    Plasmid
    #61972
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
  38. pGMC00010 (aka pRC0495)

    Plasmid
    #195295
    Purpose
    Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP
    Depositor
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  39. pLKO.1-blast shGFP

    Plasmid
    #110419
    Purpose
    shGFP control, silence GFP gene, blasticidin selection.
    Insert
    GFP
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA PolIII)
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  40. pCGN-Sos Cat

    Plasmid
    #32917
    Depositor
    Insert
    SOS1 (SOS1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Contains only aa 564-1049
    Promoter
    CMV
    Available since
    Jan. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
  41. 2NVR

    Plasmid
    #51340
    Purpose
    Catalytic domain of human histone deacetylase HDAC7
    Depositor
    Insert
    HDAC7
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  42. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lac0
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
  43. p3E_he1a:ECFP

    Plasmid
    #113880
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    ECFP
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  44. pCDH-EF1-Nluc

    Plasmid
    #73024
    Purpose
    Express Nluc reporter under EF1 promoter
    Depositor
    Insert
    Nluc
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
  45. pTRE2-Hyg-EGFP

    Plasmid
    #177152
    Purpose
    EGFP for the tet-off system
    Depositor
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  46. pAAV-CAG-Kaede

    Plasmid
    #219433
    Purpose
    AAV expression vector for Kaede
    Depositor
    Insert
    Kaede
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 2351 - 2400 of 8867 results