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We narrowed to 2,481 results for: D10

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Showing: 941 - 960 of 2481 results

  4. ABE-RA3.0

    Plasmid
    #208301
    Purpose
    Expresses ABE-RA3.0 in mammalian cells
    Depositor
    Weixin Tang
    Publication
    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-RA3.0-SpCas9 D10A
    Expression
    Mammalian
    Mutation
    E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    208301-plasmid-map-sequence-id-410368

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  5. pMOD_A1810

    Plasmid
    #91040
    Purpose
    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSP
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-P2A-TaCas9_dead (D10A + H840A)
    Use
    CRISPR
    Mutation
    D10A, H840A
    Promoter
    ZmUbi
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only

  7. pLenti PGK mKate2-OGT D554N (Puro)

    Plasmid
    #154292
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)
    Depositor
    Suzanne Walker
    Publication
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    D554N mutation (Ser/Thr glycosylation-incompetent)
    Promoter
    PGK
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    154292-plasmid-map-sequence-id-332434

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  8. LADL Anchor

    Plasmid
    #127664
    Purpose
    Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter
    Depositor
    Jennifer Phillips-Cremins
    Publication
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-CIBN-2A-Puro
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    Cas9 (D10A,H840A) mutant
    Promoter
    EF1a
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127664-plasmid-map-sequence-id-247037

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  9. p3s-ABE7.10

    Plasmid
    #113128
    Purpose
    expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized
    Depositor
    Jin-Soo Kim
    Publication
    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE 7.10
    Tags
    HA epitope and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    113128-plasmid-map-sequence-id-216920

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  15. pMOD_A0801

    Plasmid
    #91022
    Purpose
    Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-P2A-AtCas9_dead (D10A + H840A)
    Use
    CRISPR
    Mutation
    D10A, H840A
    Promoter
    35S
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91022-plasmid-map-sequence-id-181775

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  16. Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

    Plasmid
    #24419
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Expresses mCherry and Rs1 via P2A ribosomal skip sequence. Expresses tTA under EF1α promoter. Rosa26 homology arms for integration.
    Depositor
    Edward Hsiao
    Publication
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Inserts
    RS1 (HTR4 Human)
    tTA
    Use
    Mouse Targeting
    Tags
    FLAG and mCherry-P2A
    Mutation
    D100A
    Promoter
    EF1α and TRE, miniCMV
    Available since
    March 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    24419-plasmid-map-sequence-id-11416

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  17. VP64-dCas9

    Plasmid
    #177171
    Purpose
    VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VP64-dCas9(D10A, H840A)
    Use
    CRISPR
    Tags
    NLS and NLS-3xFLag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177171-plasmid-map-sequence-id-348230

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  19. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161817-plasmid-map-sequence-id-320651

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Showing: 941 - 960 of 2481 results