Addgene

We narrowed to 4,075 results for: PRS

Showing: 941 - 960 of 4075 results

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pRSET-triple mutation-pa-GCaMP6f

Plasmid

#67570

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

pa-GCaMP6f

Use

Tags

HIS-tag

Expression

Bacterial

Mutation

V115H, L64F, T65S

Promoter

T7

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pRS315-48nt-telo-I-SceI

Plasmid

#67623

Purpose

telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

3' gal1 unique sequence
ura4 (ura4 Fission Yeast)
48 bp telomere repeats
hph

Use

Yeast cen/ars plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG1-ATG17-3'UTR

Plasmid

#166848

Purpose

Expresses Atg1 with ATG17 3'UTR in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

ATG1 Promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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CT-Nano-lantern(ATP1)/pRSETB

Plasmid

#51990

Purpose

bacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Use

Tags

6xHis and chloroplast transit peptid

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pRS305:Met3p-mCherry-Tub1

Plasmid

#37553

Depositor

Wei-Lih Lee

Article

Markus et al Curr Biol. 2009 Feb 10;19(3):196-205. Ep

Insert

Met3p-mCherry-Tub1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only
pRS416 Gal TDP43 G294A

Plasmid

#27459

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Tags

Expression

Yeast

Mutation

Glycine 294 mutated to Alanine (G294A)

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pRS426 Gal-RNQ1-YFP

Plasmid

#18686

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pRS315-0nt-telo-I-SceI

Plasmid

#67624

Purpose

telomere cassette for telomere formation system containing 0 bp of S. pombe telomere repeats

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

3' gal1 unique sequence
ura4 (ura4 Fission Yeast)
telomere cassette and I-SceI cut site
hph

Use

Yeast cen/ars plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-Scs2

Plasmid

#58834

Purpose

Expresses GFP-Tagged Scs2 in yeast

Depositor

Tim Levine

Article

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Scs2 (SCS2 Budding Yeast)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(ATP1)/pRSETB

Plasmid

#51988

Purpose

bacterial expression of a Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Use

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pRS316-RGR-GFP-mHDV

Plasmid

#51058

Purpose

Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Yeast ADH1 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHH

Plasmid

#51057

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Yeast ADH1 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRS414-GPD-6xHis-Hsp82

Plasmid

#41188

Depositor

Dan Bolon

Article

Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

Insert

S. cerevisiae Hsp82

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

GPD

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pRS406-GFP-SNC1(L96V)

Plasmid

#105278

Purpose

Integrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Snc1 (SNC1 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Leucine 96 changed to Valine, intron removed

Promoter

TPI1

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRS306:Met3p-mCherry-Tub1

Plasmid

#37554

Depositor

Wei-Lih Lee

Article

Markus et al Cytoskeleton (Hoboken). 2011 Mar;68(3):1

Insert

Met3p-mCherry-Tub1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only
PSUC2-GFPantisense-CYC1t-pRS413

Plasmid

#64404

Purpose

SUC2 regulated expression of an antisense RNAi construct for GFP

Depositor

Claudia Vickers

Article

Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

Insert

GFPantisense

Use

Tags

Expression

Yeast

Mutation

Promoter

SUC2

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta13 Aphrodite

Plasmid

#13570

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta13 Aphrodite

Use

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(ATP0)/pRSETB

Plasmid

#51989

Purpose

bacterial expression of a Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP0

Use

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
7860 pRS305-pUG72-HSC82in

Plasmid

#10898

Depositor

Susan Lindquist

Article

Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

Insert

HSC82 URA3 (HSC82 Budding Yeast)

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos4a (mEosFP4a-V69T)

Plasmid

#99225

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos4a (mEos4a-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos4a

Use

Tags

Expression

Bacterial

Mutation

V69T for primed conversion applications

Promoter

T7

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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