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We narrowed to 1,100 results for: cas12a

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Showing: 941 - 960 of 1100 results

  1. pYPQ230-RRVQ

    Plasmid
    #200188
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing
    Depositor
    Yiping Qi
    Publication
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRVQ
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V, E795Q
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    200188-plasmid-map-sequence-id-396764

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  3. pTE4254

    Plasmid
    #89050
    Purpose
    Expresses St chimeric gRNA and human codon-optimized StCas9
    Depositor
    Ervin Welker
    Publication
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    St chimeric gRNA
    hStCas9
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV and human U6
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89050-plasmid-map-sequence-id-176333

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  9. pYPQ230-RR

    Plasmid
    #108860
    Purpose
    LbCpf1 Gateway entry plasmid with G548R and K611R mutations
    Depositor
    Yiping Qi
    Publication
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G548R and K611R, Cpf1 is rice codon optimized
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108860-plasmid-map-sequence-id-209932

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  12. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

    Plasmid
    #140198
    Purpose
    Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a(D156R)-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNase deactivated, D156R for improved activ…
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140198-plasmid-map-sequence-id-309713

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  13. pCSN074

    Plasmid
    #166731
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Publication
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166731-plasmid-map-sequence-id-332203

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  14. pJK503

    Plasmid
    #155380
    Purpose
    dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available since
    May 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    155380-plasmid-map-sequence-id-309009

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  18. pY116 (pcDNA3.1-huMb2Cpf1)

    Plasmid
    #92292
    Purpose
    Expression of humanized Mb2Cpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huMb2Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92292-plasmid-map-sequence-id-181491

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  19. pYPQAT51

    Plasmid
    #223423
    Purpose
    T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-LbCas12a-RRV-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223423-plasmid-map-sequence-id-453500

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Showing: 941 - 960 of 1100 results