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pF144 pAcGFP1 SOD1G85R

Plasmid

#26405

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

G85R

Promoter

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
pF143 pAcGFP1 SOD1G37R

Plasmid

#26404

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

G37R

Promoter

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3_nmPKA-CαN-mPAGFP

Plasmid

#114130

Purpose

Mammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation (Prkaca Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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piGECI-N1

Plasmid

#160421

Purpose

Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL3-pCMV7.1

Plasmid

#155021

Purpose

N-terminal 3xFLAG-tagged CUL3 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 3 (CUL3 Human)

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

pCMV7.1

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf46

Plasmid

#12734

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf46

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Neo(+) hSUMF1

Plasmid

#24386

Depositor

Carolyn Bertozzi

Article

Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

Insert

hSUMF1 (SUMF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX4T-E2-25K

Plasmid

#63572

Purpose

Expression of human Ubiquitin conjugating enzyme E2-25K

Depositor

Titia Sixma

Article

Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

Insert

UBE2-25K (UBA7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Isoform 1 (200 resiudes)

Promoter

tac

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pUC Ad VAI

Plasmid

#175518

Purpose

For mammalian expression of adenovirus VAI RNA

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

Ad VAI RNA

Use

Adenoviral

Tags

Expression

Mutation

Promoter

lac promoter

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1-HA

Plasmid

#24399

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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XMRV vp62

Plasmid

#26189

Depositor

Joseph DeRisi

Article

Urisman et al PLoS Pathog. 2006 Mar . 2(3):e25.

Insert

XMRV VP62 (gag Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only
pQTEV-YWHAE

Plasmid

#31562

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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Munc18cwt-pDEST27

Plasmid

#85626

Purpose

GST tagged Munc18c

Depositor

David James

Article

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKC123

Plasmid

#134240

Purpose

expresses gRNA targeting CENP A 3'

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

CENP A 3' CRISPR construct

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM4Z globin NB Probe

Plasmid

#120390

Purpose

Allows in vitro SP6 Polymerase driven transcription of a beta globin northern blot probe

Depositor

Niels Gehring

Article

Voigt et al Nat Protoc. 2019 May;14(5):1603-1633. do

Insert

beta globin partial 3' UTR

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-Ctrl-sponge

Plasmid

#235152

Purpose

Expresses a EGFP-based control sponge in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/control sponge

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DD-T6B-WT-YFP

Plasmid

#235143

Purpose

Expresses the inducible T6B peptide fused with DHFR and YFP under the human synapsin promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused T6B peptide

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-pri-miR-Ctrl

Plasmid

#235158

Purpose

Expresses EGFP and a control primary transcript in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/primary transcript with control siRNA

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLAG-HA-T6B-WT-YFP

Plasmid

#235139

Purpose

Expresses the T6B peptide fused with YFP under the human synapsin promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

T6B peptide

Use

AAV

Tags

FLAG/HA

Expression

Mutation

Promoter

hSyn

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a_SMOX

Plasmid

#186345

Purpose

C-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

Insert

Spermine oxidase (SMOX Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge