We narrowed to 1,270 results for: grna cloning vector

1. pDONR221 CARD8 PAM

   Plasmid

   #169975

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   PAM

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. nAPEX2-V5-zdCas9n-3x-FLAG-GSG-linker-P2A-mNeongreen

   Plasmid

   #241191

   Purpose

   Middle entry vector for mini-Golden subcloning platform; 3x-FLAG; mNeongreen

   Depositor

   Junsu Kang

   Insert

   3x-FLAG; mNeongreen
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3x-FLAG-nzdCas9-V5-APEX2n-GSG-linker-P2A-mNeongreen

   Plasmid

   #241190

   Purpose

   Middle entry vector for mini-Golden subcloning platform; V5-APEX2n; mNeongreen

   Depositor

   Junsu Kang

   Insert

   V5-APEX2n; mNeongreen
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIREN-RetroQ-shLuc

   Plasmid

   #73666

   Purpose

   pSRIEN-RetroQ vector containing control sequence luciferase

   Depositor

   Joe Landry

   Article

   Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

   Insert

   shRNA to Luciferase
   

   Use

   Retroviral

   Available Since

   April 12, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pTGC-U6.2

   Plasmid

   #112809

   Purpose

   PCR template vector for amplifying gRNAcore-pU6.3 promoter fragment

   Depositor

   Chun Han

   Article

   Poe et al Genetics. 2018 Nov 30. pii: genetics.118

   Insert

   U6:2-gRNAcore
   

   Expression

   Bacterial

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTGC-U6.3

   Plasmid

   #112810

   Purpose

   PCR template vector for amplifying gRNAcore-pU6.2 promoter fragment

   Depositor

   Chun Han

   Article

   Poe et al Genetics. 2018 Nov 30. pii: genetics.118

   Insert

   U6:3-gRNAcore
   

   Expression

   Bacterial

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

   Plasmid

   #225754

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

   Plasmid

   #225755

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p944-SWITCH-OVER insert: EF1a-Puro

   Plasmid

   #217891

   Purpose

   insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p931-SWITCH-OVER insert: EF1a-Blasti

    Plasmid

    #217890

    Purpose

    insert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid

    #204215

    Purpose

    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Insert

    Cas13bt3 and U6.SapI sgRNA cloning site
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimisation by GenScript tool

    Promoter

    CMV/U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid

    #225756

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLC-Flag-UBE2G1 sg2R-P2A-Hygro

    Plasmid

    #124299

    Purpose

    Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    UBE2G1 (UBE2G1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    several silent mutations to disrupt sgRNA targeti…

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLC-Flag-SENP8 sg2R-P2A-Hygro

    Plasmid

    #124300

    Purpose

    Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    SENP8 (SENP8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    several silent mutations to disrupt sgRNA targeti…

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. SlugCas9

    Plasmid

    #163793

    Purpose

    Expresses SlugCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SlutrCas9

    Plasmid

    #163795

    Purpose

    Expresses SlutrCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ShaCas9

    Plasmid

    #163794

    Purpose

    Expresses ShaCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpeCas9-HF

    Plasmid

    #192141

    Purpose

    Expresses highly specific SpeCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    SpeCas9-HF
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    SpeCas9-HF (R247A, N415A, S421A)

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221 CARD8 PAM L368G

    Plasmid

    #169978

    Purpose

    pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Mutation

    PAM, L368G

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221 CARD8 PAM R394E

    Plasmid

    #169980

    Purpose

    pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Mutation

    PAM, R394E

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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