We narrowed to 18,078 results for: comp

1. pGlomyc-Ovol1

   Plasmid

   #172871

   Purpose

   Mammalian expression vector with myc-tagged mouse Ovol1 coding sequence

   Depositor

   Renee van Amerongen

   Article

   Mourao et al bioRxiv 2021.06.22.449461

   Insert

   Ovol1 (Ovol1 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-RanGAP*

   Plasmid

   #175237

   Purpose

   Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   RANGAP1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Amino acid 562 F to A

   Promoter

   tac

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-FKBP-E2

   Plasmid

   #175243

   Purpose

   Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBE2I (UBE2I Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FKBP-EGFP-RanGAP

   Plasmid

   #175236

   Purpose

   Bacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   RANGAP1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-anticalin-FLN-ELP-His-ybbr

   Plasmid

   #168039

   Purpose

   Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFL GST-UBXN2A

   Plasmid

   #169016

   Purpose

   Expresses GST-UBXN2A (human) in insect cells

   Depositor

   Hemmo Meyer

   Article

   Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

   Insert

   UBXN2A (UBXN2A Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-SdrG-FLN-ELP-His-ybbr

   Plasmid

   #168047

   Purpose

   Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Use

   Tags

   3xELP linker, 6x Histag, ddFLN4, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-MIOX only

   Plasmid

   #166683

   Purpose

   Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   GFP-MIOX
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTEF1-UDH

   Plasmid

   #166679

   Purpose

   Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   UDH
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. wtVP1 only

    Plasmid

    #166673

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221-kcnma1a

    Plasmid

    #164951

    Purpose

    Zebrafish kcnma1a gene CDS

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

    Insert

    kcnma1a (kcnma1a Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNG1304

    Plasmid

    #162488

    Purpose

    Expresses HelD K239A from Bacillus subtillis

    Depositor

    Peter Lewis

    Article

    Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

    Insert

    HelD (helD Bacillus subtillis 168)
    

    Use

    Tags

    6His-V5

    Expression

    Bacterial

    Mutation

    Changed Lysine 239 to Alanine (K239A)

    Promoter

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-MS2-HA-HsNot4_275-575_AG

    Plasmid

    #148752

    Purpose

    Mammalian Expression of HsNot4_275-575

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    HsNot4_275-575 (CNOT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEBTetDBl-CLIP-HsSAS6

    Plasmid

    #136839

    Purpose

    Mammalian expression of the centrosomal protein SAS6 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-SAS6 (SASS6 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEBTetBl-CLIP-Sfi1

    Plasmid

    #136842

    Purpose

    Mammalian expression of the centrosomal protein hSfi1 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-hSfi1 (SFI1 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEBTetBl-CLIP-HsSAS6

    Plasmid

    #136838

    Purpose

    Mammalian expression of the centrosomal protein SAS6 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-SAS6 (SASS6 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEBTetD-SNAP-HsSAS6

    Plasmid

    #136790

    Purpose

    Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-SAS6 (SASS6 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p5E-161K enhancer-NR2F2-MUT

    Plasmid

    #138371

    Purpose

    Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutated

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    161k human enhancer with conserved NR2F2 binding sites mutated
    

    Use

    Gateway compatible

    Tags

    Expression

    Mutation

    NR2F2 sites mutated

    Promoter

    none

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p5E-161K enhancer-ETS-CORE-MUT

    Plasmid

    #138372

    Purpose

    Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutated

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    human 161K human enhancer with ETS bind sites mutated
    

    Use

    Gateway compatible

    Tags

    Expression

    Mutation

    ETS factor binding sites mutated

    Promoter

    NONE

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIGc-Xph18

    Plasmid

    #133029

    Purpose

    Expresses Xph18 in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph18
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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