We narrowed to 29,899 results for: des.2

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pLentiCRISPRv1.linker_KO

Plasmid

#164688

Purpose

For CRISPR knockout of miR-144~451 linker region by lentiviral delivery of Cas9 and linker gRNA

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-144~451 linker CRISPR KO gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pXL-CAG-mBirA272-NRX3b

Plasmid

#43921

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

BirA272-NRX3b (birA Human, Synthetic)

Tags

2XFLAG

Expression

Mammalian

Mutation

See comments

Promoter

CAG

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pJ291_WT_low

Plasmid

#128855

Purpose

Low copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPS1601

Plasmid

#8921

Depositor

Pamela Silver

Article

Valentini et al Genetics. 2002 Feb . 160(2):393-405.

Insert

PAB1 (PAB1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pGAD424-rSyntenin1

Plasmid

#89425

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP

Tags

Gal4 AD

Expression

Yeast

Promoter

ADH1

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSox2-bd_mFGF_eGFP

Plasmid

#34704

Depositor

Hollis Cline

Article

Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

Insert

eGFP

Use

Xenopus expression

Tags

165-bp minimal FGF4 promoter and 6x Sox2/Oct3-4 t…

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pNCMV 16E6no* Y54D

Plasmid

#47705

Purpose

Defective for p53 binding

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 16 E6no* (E6 HPV)

Tags

FLAG and HA

Expression

Mammalian

Mutation

del first 7 aa,Y54D, V42L*

Promoter

CMV

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only
pJ211_rha_WT_med

Plasmid

#128852

Purpose

Medium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBC2102-reci

Plasmid

#117171

Purpose

U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_wtCas9 without NLS

Use

Lentiviral

Promoter

U6 Promoter

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBP-TL9

Plasmid

#74098

Purpose

TL9 RBS (AAAAAG)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

TL9 RBS

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pXL-CAG-mBirA272-NRX3b D137A

Plasmid

#43922

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

BirA272-NRX3b (birA Human, Synthetic)

Tags

2XFLAG

Expression

Mammalian

Mutation

D137A

Promoter

CAG

Available Since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0007

Plasmid

#117534

Purpose

Level0 Golden Gate part: CDS, LbCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

LbCas12a (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_UG7

Plasmid

#135990

Purpose

Expresses UG7 in mammalian cells.

Depositor

Sumio Terada

Article

Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

Insert

UtrCH

Tags

helical linker-mEGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPK720

Plasmid

#38147

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

dpy-7p::ptc-3::gfp (ptc-3 )

Tags

EGFP

Expression

Worm

Promoter

dpy-7

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pUbiSXR

Plasmid

#64403

Purpose

Ubiquitin promoter driving synthetic xylanase (sXynA) gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.

Depositor

Claudia Vickers

Article

Vickers et al Plant Cell Rep. 2003 Sep;22(2):135-40. E

Insert

xylanase

Expression

Plant

Mutation

codon optimized for plant expression

Promoter

Plant ubiquitin (Ubi-1) promoter (with untranslat…

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAP608-1

Plasmid

#99484

Purpose

H2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTR

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

H2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pPK348

Plasmid

#38145

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

ptc-3::GFP (ptc-3 Nematode)

Expression

Worm

Promoter

ptc-3

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pESC-LEU2-RAD30cmyc

Plasmid

#60821

Purpose

to overexpress yeast polymerase eta in yeast

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Mutat Res. 2001 Jul 1;478(1-2):129-39.

Insert

RAD30 (RAD30 Budding Yeast)

Tags

c-myc

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
Xp75 DDD

Plasmid

#61282

Purpose

Expresses Xenopus p75 DDD domain

Depositor

Mark Bothwell, Lara Hutson

Article

Hutson et al J Neurobiol. 2001 Nov 5;49(2):79-98.

Insert

p75 DDD (p75NTRa Frog)

Use

Xenopus expression

Expression

Mammalian

Mutation

lacks the putative death domain (contains amino a…

Promoter

CMV

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only