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pMRMTK-clo5

Plasmid

#203956

Purpose

Cloning plasmid containing the chloramphenicol resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Chloramphenicol Resistance Gene

Expression

Plant

Promoter

CAT promoter

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo7

Plasmid

#203958

Purpose

Cloning plasmid containing the ampicillin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Ampicillin Resistance Gene

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo8

Plasmid

#203959

Purpose

Cloning plasmid containing the spectinomycin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Spectinomycin Resistance Gene

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo9

Plasmid

#203960

Purpose

Cloning plasmid containing the tetracycline resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Tetracycline Resistance Gene

Expression

Plant

Promoter

CAT promoter

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo3

Plasmid

#203921

Purpose

Cloning plasmid containing the ColE1 origin insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

ColE1 Origin of Replication

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo3'

Plasmid

#203924

Purpose

Cloning plasmid containing the ColE1 origin insert with an ampicillin resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

ColE1 Origin of Replication

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pZG164

Plasmid

#202583

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4 AvrRpm1

Plasmid

#209185

Purpose

pENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type III effector AvrRpm1 without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AvrRpm1

Use

Gateway-compatible entry vector

Promoter

none

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PunyEEER

Plasmid

#208326

Purpose

C-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PunyEEER

Tags

PunyEEER

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPI230

Plasmid

#207431

Purpose

Safe haven knock-in plasmid for the act5 locus for C-terminal mNeonGreen fusions

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

C-terminal mNeonGreen

Use

Unspecified

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPI231

Plasmid

#207432

Purpose

Safe haven knock-in plasmid for the act5 locus for N-terminal mNeonGreen fusions

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

N-terminal mNeonGreen

Use

Unspecified

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPI214

Plasmid

#207440

Purpose

ErkB knock out vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Modular BE4max for GG insertion

Plasmid

#198891

Purpose

Easily insert deaminase of choice into BE4max using Golden Gate cloning

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

CRISPR associated protein 9 (cas9 Streptococcus pyogenes)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.cc81-mut

Plasmid

#199745

Purpose

Expresses AAV2 Rep and AAV.cc81 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV.cc81 Cap

Use

AAV

Expression

Mammalian

Mutation

mutated amino acids 586-592 in Cap

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.cc84-mut

Plasmid

#199746

Purpose

Expresses AAV2 Rep and AAV.cc84 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV.cc84 Cap

Use

AAV

Expression

Mammalian

Mutation

mutated amino acids 586-592 in Cap

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pZG163

Plasmid

#202582

Purpose

RNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE-Per_sig_pep

Plasmid

#202152

Purpose

Level 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Peroxisome signal peptide

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 19, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pZCS41 (U6p::GCGAAGTGACGGTAGACCGT)

Plasmid

#193050

Purpose

Encodes guide RNA expression targeting PX740 landing pad

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

Guide RNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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MLKP1delKASHec

Plasmid

#130845

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Mutation

Deletion of KASH domain, addition of three alanin…

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKG1delKASHec

Plasmid

#130846

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Mutation

Deletion of KASH domain

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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