We narrowed to 59,498 results for: %s

1. pSKC11 - UTRQ

   Plasmid

   #110490

   Purpose

   Monitor the expression of Luciferase driven by the NRAS 5'-UTR.

   Depositor

   Shankar Balasubramanian

   Article

   Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

   Insert

   NRAS 5'-UTR (bp 1-254) (NRAS Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Promoter

   T7

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLR5-CAG-TetR_W43F-3xmNG

   Plasmid

   #174880

   Purpose

   PiggyBac transposon vector for expression of TetR(W43F)-3x-mNeonGreen

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   TetR(W43F)-3xmNeonGreen
   

   Use

   Piggybac vector

   Expression

   Mammalian

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

   Plasmid

   #85041

   Purpose

   This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   loxP-stop-loxP
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Lyn-PlcR(K30/32L)

   Plasmid

   #181831

   Purpose

   Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-PlcR (K30L,K32L)
   

   Tags

   ECFP, EYFP, and N-terminal targeting sequence fro…

   Expression

   Mammalian

   Mutation

   Lysines 30 and 32 in PH domain mutated to leucine.

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV126

   Plasmid

   #63192

   Purpose

   pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-JNKKTRmRuby2

   Plasmid

   #59148

   Purpose

   ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   JNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
   

   Tags

   mRuby2

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG mEmerald-LAMP1

   Plasmid

   #168513

   Purpose

   Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

   Depositor

   Franck Polleux

   Article

   Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

   Insert

   LAMP1 (Lamp1 Rat)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

   Plasmid

   #107320

   Purpose

   Expresses SpCas9 fused to SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 is fused to SaCas9

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLR5-CAG-NLS-SNAP

   Plasmid

   #174879

   Purpose

   PiggyBac transposon vector for expression of NLS-SNAP

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   NLS-SNAP
   

   Use

   Piggybac vector

   Expression

   Mammalian

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTYF-super-mfSST-0.3k-Venus-WPRE

    Plasmid

    #156393

    Purpose

    Expresses Venus under SST gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. SB_concave

    Plasmid

    #110102

    Purpose

    pBXPHM3 with scaffold Sybody of the concave library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the concave library
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE81L-KOFP7

    Plasmid

    #160330

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2020 May 27;68(21):58

    Insert

    KOFP7
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TaRGET-ABE-C3.1 ver4.1

    Plasmid

    #188251

    Purpose

    Hypercompact adenine base editors

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Chem Biol. 2022 Sep;18(9):1005-1013.

    Insert

    TaRGET-ABE3.1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBT311_pH11-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

    Plasmid

    #52544

    Purpose

    Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attPx3 (70 bp each)
    Neo
    PhiC31o integrase
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Shp2 biosensor

    Plasmid

    #134346

    Purpose

    FRET biosensor. To visualize Shp2 activity by FRET

    Depositor

    Yingxiao Wang

    Article

    Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

    Insert

    Shp2 Biosensor
    

    Expression

    Mammalian

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-mH6-Cas12c2

    Plasmid

    #120873

    Purpose

    Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12c2
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcFLAG-CSK-WT

    Plasmid

    #74502

    Purpose

    Overexpression of CSK in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

    Insert

    CSK (CSK Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K222R

    Promoter

    CMV

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pTYF-super-mfCCK-0.5k-Venus-WPRE

    Plasmid

    #156392

    Purpose

    Expresses Venus under CCK gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-NICD1

    Plasmid

    #46048

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Entry vector

    Tags

    Myc tag

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

20. SB_convex

    Plasmid

    #110104

    Purpose

    pBXPHM3 with scaffold Sybody of the convex library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the convex library
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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