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Showing: 9661 - 9680 of 65831 results

  4. pUSE-Src-YF-UniRapR-mCerulean-myc

    Plasmid
    #45381
    Purpose
    Mammalian expression of constitutively active Src kinase (YF mutant) fused to uniRapR, which switches on activity in the presence of rapamycin. mCerulean for fluorescence visualization.
    Depositor
    Klaus Hahn
    Publication
    Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Src-YF-UniRapR-mCerulean-myc
    Tags
    Myc, Myr, and mCerulean
    Expression
    Mammalian
    Mutation
    changed mSrc Tyrosine 529 to Phenylalanine
    Promoter
    CMV
    Available since
    Nov. 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    45381-plasmid-map-sequence-id-67600

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  9. mCherry-CAD

    Plasmid
    #73566
    Purpose
    Soluble red fluorescent activator of Orai1 channels
    Depositor
    Michael Cahalan
    Publication
    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-CAD (STIM1 342-448) (STIM1 Human, Synthetic)
    Tags
    6x His and mCherry
    Expression
    Mammalian
    Promoter
    CMV Immediate Early
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    73566-plasmid-map-sequence-id-137397

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  12. HSPA9A

    Plasmid
    #42469
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSPA9A (HSPA9 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187950
    Purpose
    ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187950-plasmid-map-sequence-id-373501

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  16. dCas9-CBP HAT

    Plasmid
    #179547
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179547-plasmid-map-sequence-id-354768

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  17. dCas9-p300 PHD-HAT

    Plasmid
    #179545
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179545-plasmid-map-sequence-id-354761

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  19. pJ137

    Plasmid
    #29487
    Depositor
    Jonathan Tait
    Publication
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available since
    May 17, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 9661 - 9680 of 65831 results