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pEN_Tmcs

Plasmid

#25751

Purpose

Entry vector with TRE promoter followed by multiple cloning site.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pVP91A-pcaHG

Plasmid

#113766

Purpose

Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.

Depositor

John Lipscomb

Publication

Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11

Inserts

β subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase

Tags

Met-6xHis

Expression

Bacterial

Promoter

T5 promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-Ef1a-Con/Fon-BFP

Plasmid

#137129

Purpose

Intersectional viral expression of BFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eYFP

Plasmid

#117383

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has service

AAV1

Insert

EYFP

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMKO shRNA Bim

Plasmid

#17235

Depositor

Joan Brugge

Publication

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bim shRNA (BCL2L11 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pPRIME-CMV-dsRed-recipient

Plasmid

#11658

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling dsRed expression

Depositor

Stephen Elledge

Publication

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

dsRed

Expression

Mammalian

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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eSrtA(4S-9) pET29b

Plasmid

#75146

Purpose

reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

Depositor

David Liu

Publication

Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

reprogrammed sortase A variant

Tags

6x His

Expression

Bacterial

Mutation

N98D S102C A104V A118T F122A K134R F144L I182V E1…

Promoter

T7

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCDNA3 Flag MKK7a1 (glu)

Plasmid

#14540

Depositor

Roger Davis

Publication

Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MKK7a1(Glu) (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Ser-198Glu Thr-202Glu

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
YFP NLS Beta-Actin S14C

Plasmid

#60614

Purpose

Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

Depositor

Primal de Lanerolle

Publication

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

S14C

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSG 25

Plasmid

#63867

Purpose

Expression of beta-galactosidase at high levels in E. coli

Depositor

Albert Dahlberg

Publication

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
U6-shRNA pgkCre

Plasmid

#24971

Depositor

Tyler Jacks

Publication

Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 10

Insert

shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV.CAG.(mito).cpSFGFP.HaloTag

Plasmid

#214925

Purpose

Expresses mitochondrially targeted non-responsive control

Depositor

Timothy Ryan

Publication

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).cpSFGFP.HaloTag

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779)

Plasmid

#199776

Purpose

enhancer AAV for Cre recombinase expression in striatal direct pathway D1-MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has service

AAV PHP.eB

Insert

iCre(R297T)

Use

AAV

Mutation

R297T for reduced recombinase efficiency.

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJ136

Plasmid

#29486

Depositor

Jonathan Tait

Publication

Jin et al J Biol Chem 2004; 279:40351-57.

Insert

annexin V-128 (ANXA5 Human)

Tags

(Met)-Ala-Gly-Gly-Cys-Gly-His

Expression

Bacterial

Mutation

Deletion of the initiator Met at position 1 of wi…

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only
XP12 Xbra

Plasmid

#16978

Depositor

Randall Moon

Insert

Xbra (t.S Frog)

Expression

Bacterial

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
TRE-mCherry-p2A-eNpHR3.0-TS

Plasmid

#158703

Purpose

Reporter construct

Depositor

Alice Ting

Publication

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

mCherry-p2A-eNpHR3.0-TS

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

TRE

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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hGATA-1 WT

Plasmid

#118352

Purpose

This plasmid expresses human GATA-1 WT.

Depositor

Lea Sistonen

Publication

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hGATA-1 (GATA1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEntr-Tbx5flbio

Plasmid

#32968

Depositor

William Pu

Publication

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

Tbx5 (TBX5 Human)

Use

Gateway Cloning

Tags

flag-bio

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAWp28

Plasmid

#73850

Purpose

pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Timothy Lu

Publication

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Type

Empty backbone

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pXL003-ishRNA-beta-catenin-2

Plasmid

#36299

Depositor

Sean Palecek

Publication

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-2 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Promoter

TetO-H1

Available since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only