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Showing: 9701 - 9720 of 9763 results

  1. Anti-GABA-A-R-Alpha4 [N398A/34R-2b]

    Plasmid
    #220432
    Purpose
    Mammalian Expression Plasmid of anti-GABA-A-R-Alpha4 (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N398A/34.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-GABA-A-R-Alpha4 (Rattus norvegicus) recombinant mouse monoclonal antibody. (Gabra4 Mouse)
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    220432-plasmid-map-sequence-id-435313

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  4. Anti-Cavbeta4 Ca2+ channel [N10/7R-2b]

    Plasmid
    #219473
    Purpose
    Mammalian Expression Plasmid of anti-Cavbeta4 Ca2+ channel (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N10/7.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Cavbeta4 Ca2+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody. (Cacnb4 Mouse)
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219473-plasmid-map-sequence-id-431047

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  5. pcDNA3.1/hFGFR3-K650M-V5

    Plasmid
    #214909
    Purpose
    expression of the K650M mutant variant of human FGFR3, which is associated with Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans (SADDAN)
    Depositor
    Pavel Krejčí
    Article
    Kunova Bosakova et al Proc Natl Acad Sci U S A. 2019 Feb 19. p
    Insert
    FGFR3 (FGFR3 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    K650M substitution
    Promoter
    CMV
    Available Since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214909-plasmid-map-sequence-id-426045

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  7. Anti-KCNT1/Slo2.2/Slack K+ channel [N3/26R-1]

    Plasmid
    #211599
    Purpose
    Mammalian Expression Plasmid of anti-KCNT1/Slo2.2/Slack K+ channel (Rat) subclass-switched IgG1 R-mAb. Derived from hybridoma N3/26.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-KCNT1/Slo2.2/Slack K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody. (Kcnt1 Mouse)
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    Jan. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    211599-plasmid-map-sequence-id-418372

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  8. pαH-S-GSAS-2P-N317P

    Plasmid
    #196378
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:
    Insert
    S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  9. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔA LS

    Plasmid
    #194926
    Purpose
    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Large Subunit Region Val 177 to Arg 263
    Depositor
    Hannah Rabinowich
    Article
    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649
    Insert
    N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Val 177 to Arg 263 (Casp9 Mouse)
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Cysteine 325 to Alanine to Eliminate Prot…
    Promoter
    CMV
    Available Since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194926-plasmid-map-sequence-id-384890

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  10. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔLD + ΔSS

    Plasmid
    #194610
    Purpose
    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Linker + Small Subunit Domain
    Depositor
    Hannah Rabinowich
    Article
    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649
    Insert
    N-terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Ala 354 to Ser 454 (Casp9 Mouse)
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Cysteine 325 to Alanine to eliminate prot…
    Promoter
    CMV
    Available Since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194610-plasmid-map-sequence-id-384887

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  11. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

    Plasmid
    #192784
    Purpose
    Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192784-plasmid-map-sequence-id-379372

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  12. pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)

    Plasmid
    #192704
    Purpose
    Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPm…
    Expression
    Mammalian
    Mutation
    meGFPm: gluatmic acid 142 to asparagine and histi…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192704-plasmid-map-sequence-id-379659

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  16. GFP-Cstem(7A)-Tac(5A)

    Plasmid
    #162499
    Purpose
    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    CD8a stem region with mutations and Tac full length with mutated stem region (IL2RA, CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five theronine and two serine residues are mu…
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162499-plasmid-map-sequence-id-323491

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  18. Gs(short)-CASE

    Plasmid
    #168124
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG1
    Expression
    Mammalian
    Mutation
    NLuc is inserted at N136/V137 within GNAS
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168124-plasmid-map-sequence-id-332868

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  19. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

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  20. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107943-plasmid-map-sequence-id-207614

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Showing: 9701 - 9720 of 9763 results