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We narrowed to 63,769 results for: cloning

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Showing: 9701 - 9720 of 63769 results

  2. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  3. pGD283

    Plasmid
    #129733
    Purpose
    Destination plasmid containing the 35S:XVE estradiol inducible driver and the A-G overhangs for GreenGate cloning of an expression casette for estradiole-inducible expression in plants.
    Depositor
    Rüdiger Simon
    Publication
    Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d
    Type
    Empty backbone
    Expression
    Plant
    Available since
    Oct. 31, 2019
    Availability
    Academic Institutions and Nonprofits only
    129733-plasmid-map-sequence-id-258677

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  5. pCfB2189

    Plasmid
    #67532
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Publication
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67532-plasmid-map-sequence-id-116416

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  6. pMDC32B-OsMIR390-B/c

    Plasmid
    #61467
    Purpose
    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.
    Depositor
    James Carrington
    Publication
    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1
    Insert
    OsMIR390-B/c
    Expression
    Plant
    Mutation
    O. sativa MIR390 precursor sequence including a c…
    Promoter
    2x35S
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61467-plasmid-map-sequence-id-100060

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  9. pDosa2

    Plasmid
    #260656
    Purpose
    Updated cloning vector to express a fusion of a variant protein and a tRNA modifying enzyme TrmA from Pseudomonas thermotolerans, to covalently link an RNA barcode
    Depositor
    Stan Fields
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    Aug. 25, 2026
    Availability
    Academic Institutions and Nonprofits only
    260656-plasmid-map-sequence-id-518995

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  12. Hs.KRAS4b G13D

    Plasmid
    #83133
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    G13D
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83133-plasmid-map-sequence-id-158096

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  13. POLG-pcDNA3.1

    Plasmid
    #191487
    Purpose
    Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.
    Depositor
    Hans Zempel
    Insert
    POLG (POLG Human)
    Tags
    V5 tag, 6X His
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    191487-plasmid-map-sequence-id-415364

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  18. pY036_ATP1A1_G3_Array

    Plasmid
    #86619
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86619-plasmid-map-sequence-id-168855

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Showing: 9701 - 9720 of 63769 results