We narrowed to 60,275 results for: cloning

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pMuLE ENTR U6-miR-30 L1-R5

Plasmid

#62113

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3

Plasmid

#62131

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

CRISPR; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9

Plasmid

#87917

Purpose

Gateway cloning

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgHTT1, sgGFP2, shCas9

Expression

Mammalian

Promoter

H1, U6, 7SK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A14

Plasmid

#132105

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

Insert

SLC39A14 (SLC39A14 Human)

Expression

Mammalian

Available Since

Dec. 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC7A3

Plasmid

#132301

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

Insert

SLC7A3 (SLC7A3 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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p3E-DrCav1

Plasmid

#194290

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

caveolin1 (cav1.S Frog)

Use

Multisite gateway entry vector

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pMEL14

Plasmid

#107920

Purpose

LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4

Plasmid

#62128

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

CRISPR; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-NL63-Envelope

Plasmid

#168924

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-Envelope (HCNV63gp4 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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DYRK2_HUMAN_D0

Plasmid

#79729

Purpose

This plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK2 (DYRK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-AviMta3-3xFiBsd

Plasmid

#140962

Purpose

full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis associated 3 (Mta3 Synthetic, Mouse)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-G2ZYP2

Plasmid

#103110

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-nsp8

Plasmid

#168957

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp8 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-nsp8

Plasmid

#168897

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp8 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-1-nsp8

Plasmid

#168850

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-nsp8 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-nsp8

Plasmid

#168821

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp8 (orf1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR E2 HSPter-RPS5Ap-mito

Plasmid

#163146

Purpose

Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4; Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORF

Depositor

Shin-ichi Arimura

Article

Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

Insert

A terminator of Arabidopsis heat shock protein, RPS5A promoter, and mitochondrial presequence

Use

Entry vector

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-Q927P4

Plasmid

#103131

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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Nup98-pDONR221

Plasmid

#124241

Purpose

Gateway entry clone of Drosophila Nup98-96 cDNA.

Depositor

Peter Vilmos

Article

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Nup98-96 (Nup98-96 Fly)

Expression

Insect

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAF138

Plasmid

#135923

Purpose

All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCitrine fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

Depositor

Andrew S. Flies

Article

Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

Insert

TNFRSF9 fused to mCitrine

Use

Transposon

Tags

mCitrine

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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