We narrowed to 18,496 results for: Met

1. pJCC_084 Cas12i1 D647A

   Plasmid

   #171671

   Purpose

   For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   Cas12i1 D647A
   

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed aspartate 647 to alanine

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-EGFP-DmMe31B_268-459_D

   Plasmid

   #146119

   Purpose

   Insect Expression of DmMe31B_268-459

   Depositor

   Elisa Izaurralde

   Article

   Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

   Insert

   DmMe31B_268-459 (me31B Fly)
   

   Expression

   Insect

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r

   Plasmid

   #162936

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Triple FLAG epitope tag fusion, AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. C512-E01: attB5r-meGFP-nostop-attB1r

   Plasmid

   #162940

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   monomeric enhanced Green fluorescent protein
   

   Use

   Synthetic Biology

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r

   Plasmid

   #162937

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6 fusion, AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. C514-E11: attB5r-RU5u-His6-nostop-attB1r

   Plasmid

   #162953

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6 purification tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C453-E25: attB4-mPol2p>-attB5

   Plasmid

   #162981

   Purpose

   Gateway attB4/attB5 entry clone for mammalian expression using the murine RNA polymerase 2 promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   murine RNA polymerase 2 promoter
   

   Use

   Synthetic Biology

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r

   Plasmid

   #162956

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-Halotag fusion tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r

   Plasmid

   #162935

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Maltose-binding protein fusion, AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. C453-E09: attB4-Grp78p>-attB5

    Plasmid

    #162974

    Purpose

    Gateway attB4/attB5 entry clone for mammalian expression using the human Grp78 promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    human Glucose response protein 78 promoter
    

    Use

    Synthetic Biology

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C514-E09: attB5r-RU5u-FLAG-nostop-attB1r

    Plasmid

    #162951

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    FLAG epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. C514-E59: attB5r-RU5u-2xHA-nostop-attB1r

    Plasmid

    #162966

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Dual HA epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C514-E42: attB5r-RU5u-Strep2-nostop-attB1r

    Plasmid

    #162961

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Strep2 purification tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. C514-E26: attB5r-RU5u-myc-nostop-attB1r

    Plasmid

    #162960

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal Myc-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Myc epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C514-E10: attB5r-RU5u-HA-nostop-attB1r

    Plasmid

    #162952

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal HA-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    HA epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C413-E11: attB4-Grp78p>-attB1r

    Plasmid

    #162970

    Purpose

    Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    human Glucose response protein 78 promoter
    

    Use

    Synthetic Biology

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C413-E10: attB4-FerHp>-attB1r

    Plasmid

    #162969

    Purpose

    Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    human Ferritin heavy chain promoter
    

    Use

    Synthetic Biology

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C514-E24: attB5r-RU5u-V5-nostop-attB1r

    Plasmid

    #162959

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal V5-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    V5 epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C512-E34: attB5r-iRFP670-nostop-attB1r

    Plasmid

    #162947

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal iRFP670 fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    far-red iRFP670 fluorescent protein
    

    Use

    Synthetic Biology

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

    Plasmid

    #162932

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6 fusion with AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge