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pXPR_068

Plasmid

#164555

Purpose

Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

Insert

sgRNA cassette

Use

CRISPR and Lentiviral; Flp/frt

Expression

Mammalian

Promoter

Human U6

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-SHTN1L

Plasmid

#209560

Purpose

Mammalian expression of Shtn1 long isoform

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform (4930506M07Rik Mouse)

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRTP801/TA-ODD

Plasmid

#206212

Purpose

An expression vector of hypoxia-responsive transactivator under control of RTP801 promoter

Depositor

Masamichi Kamihira

Article

Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

Insert

tetracycline-responsive transactivator fused with oxygen-dependent degradation domain

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch1#1/Cre

Plasmid

#193223

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch1#1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGW045 AAV-CRISPRa base vector pAAV_OE-U6sgbbSapI(MS2)-EF1a-MPH-WPRE

Plasmid

#192161

Purpose

AAV-CRISPRa base vector

Depositor

Sidi Chen

Article

Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

Type

Empty backbone

Use

AAV

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmNanoLuc

Plasmid

#182969

Purpose

Stably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmNanoLuc

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pG.Lantern-SM50-GFP

Plasmid

#135593

Purpose

Expression of SM50 Promoter-GFP mRNA for microinjection to visualize SM50 promoter activity in S. purpuratus

Depositor

Koji Akasaka, Mamiko Yajima

Article

Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

Insert

SM50 promoter (SM50 S. purpuratus)

Use

In vitro transcription (mrna synthesis)

Tags

Green Lantern GFP

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCDC124-Halo

Plasmid

#175328

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC124 (CCDC124 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCDC86-Halo

Plasmid

#175323

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC86 (CCDC86 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-merlin 1-339

Plasmid

#168128

Purpose

Bacterial expression of human Nf2/merlin 1-339

Depositor

Tina Izard

Article

Primi et al Matrix Biol Plus. 2021 Jul 1;12:100074.

Insert

Merlin (NF2 Human)

Tags

Glutathione S-transferase (GST)

Expression

Bacterial

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pVMG0146_Cq-U6-1_2xBbsI-gRNA

Plasmid

#169238

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

Cq-U6-1_2xBbsI-gRNA

Use

CRISPR

Expression

Insect

Promoter

CPIJ039653

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-PorA-4xDQNAT

Plasmid

#128400

Purpose

Periplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

N. meningitidis PorA

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-pax3a-5.4k

Plasmid

#164966

Purpose

zebrafish pax3a -5.4k promoter

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

pax3a (pax3a Zebrafish)

Use

Gateway 5 end entry vector

Mutation

promoter -5.4k

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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SEPLuc

Plasmid

#141102

Purpose

bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc

Use

Lentiviral

Promoter

EF1α

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry-LOV

Plasmid

#135599

Purpose

Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry-LOV

Use

In vitro transcription (mrna synthesis)

Tags

LOV and mCherry

Promoter

Unknown

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K7-2xCSTREP

Plasmid

#136156

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K7

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K7

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K6-2xCSTREP

Plasmid

#136155

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K6

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K6

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K4-2xCSTREP

Plasmid

#136151

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K4.1

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K4

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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S8_attL1-N-term-EGFP-attL2

Plasmid

#128472

Purpose

EGFP entry clones

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

EGFP entry clones

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti PA-NL

Plasmid

#113453

Purpose

Lentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-NanoLuc

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

hUbC

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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