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pMXs-Sox2

Plasmid

#13367

Purpose

Retroviral expression of mouse Sox2

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

SRY-box containing gene 2 (Sox2 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pTOX2

Plasmid

#127447

Purpose

Encodes MqsR toxin. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

mqsR toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo1-NIK3x_pTRE-G418

Plasmid

#202017

Purpose

expresses LOV-Turbo1-NIK3x in the mammalian nucleolus under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-LOV-Turbo1-NIK3x

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

pTRE-Tight

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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IBMc396

Plasmid

#161954

Purpose

Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T

Expression

Bacterial

Promoter

PBAD

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-DEVD-NanoLuc

Plasmid

#98287

Purpose

caspase-3 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204816

Purpose

Bacterial expresison of Enterovirus A71 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

EV A71 3C protease

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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PE7_AAVS1_knockin_HDR_donor

Plasmid

#223001

Purpose

HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pTOX1

Plasmid

#127446

Purpose

Encodes YhaV toxin. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

yhaV toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTSK29

Plasmid

#80779

Purpose

Cloning vector with temperature sensitive origin of replication.

Depositor

Gregory Phillips

Article

Phillips et al Plasmid. 1999 Jan;41(1):78-81.

Type

Empty backbone

Available Since

Dec. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPD96_32

Plasmid

#1504

Depositor

Andrew Fire

Type

Empty backbone

Tags

IVSA, ATG-MitLS, gfpN, IVSR, gfpS65C, IVSS, IVST,…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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AW01_pU.CAG.eSpCas9(1.1).rBGpA

Plasmid

#199255

Purpose

Expression construct encoding the high specificity eSpCas9(1.1) nuclease

Depositor

Manuel Goncalves

Article

Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

Insert

High specificity eSpCas9(1.1) nuclease

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

K848A K1003A R1060A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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Tau (P301L)-VC

Plasmid

#87633

Purpose

expresses hTau fused to the C-terminal part of Venus

Depositor

Tiago Outeiro

Insert

MAPT (MAPT Human)

Tags

N-terminal part of Venus

Expression

Mammalian

Mutation

Mutation P301L

Promoter

CMV

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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L1_HyR-Ck1

Plasmid

#136125

Purpose

L1 in position 1 (next to LB in L2 construct), for HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:hptII_3t35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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UbcH5c WT pET28a

Plasmid

#12643

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)

Expression

Bacterial

Available Since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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VPS13A^mCherry

Plasmid

#118758

Purpose

VPS13A internally tagged with mCherry at residue 1372.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13A (VPS13A Human)

Tags

mCherry inserted at position 1372

Expression

Mammalian

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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mCh-Rab7A

Plasmid

#61804

Purpose

Human Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

Insert

Rab7A (RAB7A Human)

Tags

mCh

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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FKBP-NTEVp(WT)

Plasmid

#137831

Purpose

Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-NTEVp(WT)

Tags

3x FLAG and NES

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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FOP-GFP.mC

Plasmid

#35492

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

7xFOP-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

mutated 7xTCF/LEF

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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FRB-CTEVp(WT)

Plasmid

#137832

Purpose

Cytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CTEVp(WT)

Tags

3x FLAG and NES

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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