We narrowed to 34,176 results for: cel=

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pcDNA3.1-DRD2-cpHalo∆-mEGFP

Plasmid

#194679

Purpose

CMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

DRD2_cpHalo∆

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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U6-5' MS2-CircRNA

Plasmid

#213750

Purpose

For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)

Plasmid

#203907

Purpose

Encodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4LOV(SK22)-VP64-IRES-mCherry

Expression

Mammalian

Promoter

SFFV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-SA-neo-CAGGS-PE2-2A-GFP

Plasmid

#180014

Purpose

Targeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locus

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

PE2-2A-GFP

Use

CRISPR; Human genome engineering

Tags

GFP

Promoter

CAG

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmReNL

Plasmid

#182970

Purpose

Stably express PalmReNL in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmReNL

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBx1-VHH template3-3XMyc-Spacer

Plasmid

#176210

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template3-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBx1-VHH template1-3XMyc-Spacer

Plasmid

#176208

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template1-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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3xnls-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129626

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-cpmTq2-Calcium-lifetime-sensor

Tags

3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAP WT

Plasmid

#123080

Purpose

Gateway entry clone encoding human GABARAP wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Gateway Cloning

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Rabin8

Plasmid

#118070

Purpose

Expresses Flag-tagged human Rabin8 in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

human Rabin8 (RAB3IP Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinTS-A50I

Plasmid

#111831

Purpose

Vinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vector

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (A50I) (VCL Chicken)

Use

Lentiviral

Mutation

changed vinculin alanine 50 to isoleucine

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_EIF2AK2

Plasmid

#106108

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting EIF2AK2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting EIF2AK2 (EIF2AK2 Human)

Use

CRISPR

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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mPlum-CENPB-N-22

Plasmid

#55957

Purpose

Localization: Nucleus/Centromeres, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

CENPB (CENPB Human)

Tags

mPlum

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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PA-mCherry1-Vimentin-7

Plasmid

#57202

Purpose

Localization: Intermediate Filaments, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

Insert

Vimentin (VIM Human)

Tags

PA-mCherry1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEU-Gm23

Plasmid

#53738

Purpose

Used for in vitro transcription/translation of soybean His-tagged arginyl-tRNA synthetase in the Cell Free Sciences system

Depositor

Gabor Igloi

Insert

arginyl-tRNA synthetase

Use

Synthetic Biology

Tags

His tag

Promoter

SP6

Available Since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM6-C-19

Plasmid

#54171

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM6

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-CENPB-N-22

Plasmid

#54037

Purpose

Localization: Nucleus/Centromeres, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

CENPB (CENPB Human)

Tags

mEmerald

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBEST-p15A-PLlacO1-lacI-T500

Plasmid

#45393

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

lac repressor

Expression

Bacterial

Promoter

PL-lacO1

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-H-Ras_wt

Plasmid

#39509

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS (HRAS Human)

Use

Eukaryotic

Tags

HA

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pWZL Sox2 R74P

Plasmid

#26355

Depositor

Matthew Meyerson

Article

Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

Insert

Sox2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R74P

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only