163,570 results

1. pCMV-evoCAST-Cas6

   Plasmid

   #234725

   Purpose

   Engineered human-codon optimized PseCas6 in mammalian cells (for evoCAST)

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   PseCas6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-evoCAST-eCas7

   Plasmid

   #234726

   Purpose

   Engineered human-codon optimized PseCas7 in mammalian cells (for evoCAST)

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   ePseCas7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCENTR_aco_mScarlet3

   Plasmid

   #225984

   Purpose

   Arabidopsis codon-optimized mScarlet3 in an entry clone

   Depositor

   Imran Siddiqi

   Insert

   mScarlet3 Arabidopsis codon-optimized
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Arabidopsis Codon-Optimized mScarlet3

   Promoter

   Available Since

   Oct. 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. SpyCatcher003-sfGFP

   Plasmid

   #133449

   Purpose

   Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003-sfGFP
   

   Use

   Tags

   His6 and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL1142 (pSPIN, pBBR1 backbone)

   Plasmid

   #160730

   Purpose

   Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ptfLC3

   Plasmid

   #21074

   Purpose

   Mammalian expression of rat LC3 fused to mRFP and EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kimura et al Autophagy. 2007;3(5):452-60.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. sortase A pentamutant (eSrtA) in pET29

   Plasmid

   #75144

   Purpose

   evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

   Depositor

   David Liu

   Article

   Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

   Insert

   sortase A pentamutant
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T. Deleted amino a…

   Promoter

   T7

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-tevopreq1-GG-acceptor

   Plasmid

   #174038

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-AAVS1-5-1kbdonor

    Plasmid

    #234735

    Purpose

    PseCAST crRNA targeting AAVS1-5, with 1 kb donor transposon

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    AAVS1-5 crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge