We narrowed to 24,130 results for: fus

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pJBL730

Plasmid

#217374

Purpose

sfGFP reporter for Bsu yxjA 2AP riboswitch

Depositor

Julius Lucks

Article

Bushhouse et al bioRxiv 2024.03.29.587317

Insert

yxjA riboswitch sfGFP reporter

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10

Plasmid

#211466

Purpose

Lentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

mito-mCherry-GFP1-10

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211463

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-dead

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D211A, E225A, K227A

Promoter

TRE

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Mito-mEosEM

Plasmid

#132706

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mitochondria targeting sequence

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pRS416gT-GalL-Cas9

Plasmid

#79903

Purpose

pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

Depositor

Ronald Davis

Article

Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

Inserts

Cas9
guide RNA (gRNA)
Tetracycline Repressor

Use

CRISPR

Expression

Yeast

Promoter

GPM1 promoter, GalL, and RPR1 promoter with TetO …

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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mEosEM-Lamin A

Plasmid

#132707

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

lamin A

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-CMV-TFAM-BioID2-IRES-Puro

Plasmid

#211465

Purpose

Lentivirus backbone expressing human TFAM fused with BioID2

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

TFAM-BioID2

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgUpf1

Plasmid

#161810

Purpose

guide RNA for Upf1

Depositor

Yi Zhang

Article

Fu et al Sci Adv. 2020 May 29;6(22):eaay5181. doi

Insert

sgRNA targeting mouse Upf1 (Upf1 Synthetic, Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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CRY2olig-BFP-YTHDF1(D401N)-C

Plasmid

#155354

Purpose

Lentivector to express human YTHDF1(264-559) with D401N mutation and CRY2olig-BFP at the N-terminal

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1(264-559)

Use

Lentiviral

Expression

Mammalian

Mutation

D401N

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL6931

Plasmid

#217369

Purpose

sfGFP reporter for WT Pca rhtB ZTP riboswitch

Depositor

Julius Lucks

Article

Bushhouse et al bioRxiv 2024.03.29.587317

Insert

Pca rhtB riboswitch sfGFP reporter

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_hTRIM32

Plasmid

#69541

Purpose

Expresses human TRIM32 fused to c-terminal EGFP

Depositor

Martin Dorf

Article

Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

Insert

tripartite motif containing 32 (TRIM32 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pFYF1320 EGFP Site#1

Plasmid

#47511

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8HOIP

Plasmid

#50015

Purpose

Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

ring finger protein 31 (RNF31 Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF3

Plasmid

#155348

Purpose

Express full-length human YTHDF3 with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF3 (YTHDF3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1

Plasmid

#155346

Purpose

Express full-length human YTHDF1 with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (YTHDF1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF2

Plasmid

#155347

Purpose

Express full-length human YTHDF2 with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF2 (YTHDF2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

Plasmid

#211468

Purpose

Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

4xGFP11-SEC61B

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFYF1327 EGFP Site#2

Plasmid

#47512

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pFYF1328 EGFP Site#3

Plasmid

#47513

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD

Plasmid

#226967

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7346

Plasmid

#217370

Purpose

sfGFP reporter for synthetic transcriptional variant of Pca rhtB ZTP riboswitch

Depositor

Julius Lucks

Article

Bushhouse et al bioRxiv 2024.03.29.587317

Insert

Pca rhtB synthetic transcriptional riboswitch sfGFP reporter

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2441

Plasmid

#142079

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FUS (FUS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2440

Plasmid

#142944

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FUS (FUS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

Plasmid

#211469

Purpose

Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ATAD3A (ATAD3A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2-mDux

Plasmid

#138320

Purpose

Dox-inducible codon-optimized mouse Dux

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

Dux (Dux Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFYF1548 EMX1

Plasmid

#47508

Purpose

human gRNA expression vector targeting EMX1

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EMX1 (EMX1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(E502Q)

Plasmid

#226973

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) E502Q variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(E502Q)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

E502Q

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD-AE

Plasmid

#226982

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD-AE

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD_beta-gamma

Plasmid

#226981

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD_beta-gamma

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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