We narrowed to 2,115 results for: PORA
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Plasmid#169107PurposeExpresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNEU-KlacRS
Plasmid#232155PurposeSite-specific incorporation of L-lactyl-lysine into target proteins in mammalian cells.DepositorInsertKlacRS1
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mCherry-TAG-EGFP
Plasmid#232156PurposeA fluorescence reporter to show incorporation of target unnatural amino acids.DepositorInsertmCherry-linker(TAG)-EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEvol-MmKlacRS1
Plasmid#232157PurposeSite-specific incorporation of L-lactyl-lysine into target proteins in E. coli cells.DepositorInsertKlacRS1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEvol-chKlacRS-WT
Plasmid#232159PurposeA chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.DepositorInsertchKlacRS-WT
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEvol-chKlacRS-IPYE
Plasmid#232160PurposeA chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.DepositorInsertchKlacRS-IPYE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
EIF2AK2(252-551)-NanoLuc Fusion Vector
Plasmid#237172PurposeExpress EIF2AK2(252-551)-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsNanoLuc (R)ExpressionMammalianMutationTruncation of full-length protein; expressing res…PromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET MLL1/WDR5 Assay Donor Vector, MLL1
Plasmid#237139PurposeExpress MLL1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
LgBiT-KRAS 4B(G12V) BiBiT-Ready Vector
Plasmid#237076PurposeExpress LgBiT-KRAS 4B(G12V) Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsLgBiTExpressionMammalianMutationG12VPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
LgBiT-KRAS 4B G12C BiBiT Ready Vector
Plasmid#237078PurposeExpress LgBiT-KRAS 4B G12C Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsLgBiTExpressionMammalianMutationG12CPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
LgBiT-KRAS(G12D) Vector
Plasmid#237033PurposeExpress LgBiT-KRAS(G12D) Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsLgBiTExpressionMammalianMutationG12DPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HiBiT-KRAS(G12C) Fusion Vector
Plasmid#236889PurposeExpress HiBiT-KRAS(G12C) in Mammalian Cells under a CMV promoterDepositorUseTagsHiBitExpressionMammalianMutationG12CPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET Assay Vector, HaloTag-KRAS
Plasmid#236871PurposeExpress HaloTag(R)-KRAS in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET Assay Vector, NanoLuc-HRAS WT
Plasmid#236866PurposeExpress NanoLuc(R)-HRAS WT in Mammalian Cells under a CMV promoterDepositorUseTagsNanoLuc (R)ExpressionMammalianMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET Assay Vector, NanoLuc-NRAS(Q61R)
Plasmid#236863PurposeExpress NanoLuc(R)-NRAS Q61R in Mammalian Cells under a CMV promoterDepositorUseTagsNanoLuc (R)ExpressionMammalianMutationQ61RPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET Assay Vector, NanoLuc-NRAS(Q61K)
Plasmid#236854PurposeExpress NanoLuc(R)-NRAS(Q61K) in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R) and NanoLuc (R)ExpressionMammalianMutationQ61KPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoBRET Assay Vector, HaloTag-KRAS(G13D)
Plasmid#236856PurposeExpress HaloTag(R)-KRAS(G13D) in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R)ExpressionMammalianMutationG13DPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
SmBiT-KRAS4B(G12D) BiBiT-Ready Vector
Plasmid#236798PurposeExpress SmBiT-KRAS4B(G12D) Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsSmBiTExpressionMammalianMutationG12DPromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRCA595 - pBA904 Puro-T2A-mCherry
Plasmid#238171PurposeLentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertNon-targeting sgRNA
UseCRISPR and LentiviralTagsmCherryExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA594 - pBA904 Puro-T2A-BFP
Plasmid#238170PurposeLentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertNon-targeting sgRNA
UseCRISPR and LentiviralTagsmTagBFP2ExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfA
Plasmid#186411PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::venus-RfA
Plasmid#186408PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter Venus under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-CFP
Plasmid#186412PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter CFP under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-ECFP
Plasmid#186403PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-ECFP
Plasmid#186402PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::RfA-venus
Plasmid#186401PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::venus-RfA
Plasmid#186400PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::venus-RfA
Plasmid#186398PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-HA
Plasmid#186405PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-venus
Plasmid#186409PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter Venus-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HA
Plasmid#186410PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1pCNPRS
Plasmid#174719PurposetRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-5-pyridyl)alanine (pCNP), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-17
Plasmid#160717PurposeTrpC Terminator bordered by BsaI restriction sitesDepositorInserttryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-3
Plasmid#160703Purposetef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInserttef1-alpha
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k
Plasmid#127403PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
UseTagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon, W340A,…PromoterCMVAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k
Plasmid#127407PurposeExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
UseTagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: E322 to Amber codon, K305H,…PromoterCMVAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k
Plasmid#127401PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
UseTagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon, W340A,…PromoterCMVAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEVOL_PylRS(AF)-tRNA
Plasmid#223511PurposePylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codonDepositorInsertsUseTags6xHisExpressionBacterialMutationY306A; Y384FPromoteraraBAD, rrnB, and glnS and proKAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MbPylRS(DiZPK)
Plasmid#91706Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interestDepositorInsertspylS
pylT
UseTagsExpressionMammalianMutationT810G, C813T, C820G, T821C, T937A, A1046T, G1257APromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only