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Plasmid#197028PurposeAAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6DepositorInserthumanized enOsCas12f1
UseAAVTagsExpressionMutationD52R / T132RPromoterMHCK7, hU6Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPtGE35
Plasmid#107999PurposeExpresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugationDepositorInsertsOriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
I-TevI nuclease and partial linker domain
UseCRISPR and Synthetic Biology; Episomal vector for…TagsCas9ExpressionMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-YAPS94A
Plasmid#84010Purposeexpresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertYAP1 (siRNA insensitive) (YAP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationS94APromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR
Plasmid#197029PurposeAAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6DepositorInserthumanized ecDHFR-enOsCas12f1-ecDHFR
UseAAVTagsExpressionMutationD52R / T132RPromoterMHCK7, hU6Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP
Plasmid#122955PurposeLentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expressionDepositorInsertNOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
UseLentiviralTags6xhis tagExpressionMutationdeletion of EGF repeats from full length NOTCH2NLBPromoterCAG promoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB(EXPR-DS-HDAC5_HDAC3)
Plasmid#114399PurposeFor PYL-HDAC5 catalytic domain swap to HDAC3 catalytic domain expressionDepositorUseTags3xFlag-NLS (internal)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV CCTG 1200x
Plasmid#63088PurposeAdenoAssociated Virus plasmid containing expanded repeats--CCTG 1200x that correspond to Myotonic Dystrophy of type 2DepositorInsertCCUG 1200x
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI_FLAG-GFP(Y39TAG)-6His
Plasmid#223507PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
UseTags6xHis and FLAGExpressionMammalianMutationY39TAGPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV CTG 700x
Plasmid#63087PurposeAdenoAssociated Virus plasmid containing expanded repeats--CTG 700x that is the mutation found in Myotonic Dystrophic type 1DepositorInsertCTG REPEATS
UseAAVTagsEGFPExpressionMammalianMutationCTG 700x that is the mutation found in Myotonic D…PromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pMAH2-CageCys
Plasmid#71404PurposeExpresses tRNA and synthetase for mammalian encoding of a photocaged cysteineDepositorInsertaminoacyl-tRNA synthetase for a caged cysteine
UseTagsExpressionMutationPromoterCMVAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS_MmaPylT_EF1_NES-MmaPylRS WT
Plasmid#174901Purposeexpression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAG and NESExpressionMammalianMutationPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-CFP II (pMIC II)
Plasmid#52109Purposea bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorTypeEmpty backboneUseRetroviralTagsIRES-ECFPExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-DsRed FP (pMIDsRed II)
Plasmid#52110Purposea bicistronic IRES-DsRed containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorTypeEmpty backboneUseRetroviralTagsIRES-DsRedExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-YFP II (pMIY II)
Plasmid#52108Purposea bicistronic IRES-YFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorTypeEmpty backboneUseRetroviralTagsIRES-EYFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS_MmaPylT_EF1_NES-MmaPylRS AF
Plasmid#174902Purposeexpression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAG and NESExpressionMammalianMutationY306A, Y384FPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP II (pMIG II)
Plasmid#52107Purposea bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pULTRA-CNF
Plasmid#48215Purposehighly efficient suppressor plasmidDepositorInsertspolyspecific MJ tyrosyl synthetase
tRNA
UseTagsExpressionBacterialMutationY32L, L65V, F108W, Q109M, D158G, I159APromoterproK and tacIAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
iD20 LoxStopLox-Zim3-dCas9-mApple-hygro
Plasmid#204720PurposeCre-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-CMVmin:GFPmem pA
Plasmid#80823PurposeCMVmin-promoted GFPmem with pADepositorInsert3E-CMV-GFPCAAXpA spacer
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(CysCNP)RS
Plasmid#225054PurposetRNA synthetase/tRNA pair for the incorporation of S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-cysteine (CysCNPym) into proteins in response to the amber (TAG) codonDepositorArticleInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF
Plasmid#31186PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAGDepositorInsertM.j. p-azidohenylalanine RS (2 copies +tRNA)
UseTagsExpressionBacterialMutationY32T, E107N, D158P, I159L, L162Q, D286RPromoterAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-AznLRS
Plasmid#172482PurposeFor E.Coli expression, 306L309L348I384F411WDepositorInsertAznLRS and pylTcua
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-Ad5RGDPK7
Plasmid#198291PurposeProduces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions)DepositorInsertPVCpnf_pvc13-Ad5RGDPK7
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJE-Ma pylRS nitroY/haloY-F5
Plasmid#225684PurposeExpresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.DepositorInsertsM. alvus PylRS 3-NY/HaloY F5
M. alvus Pyl-tRNA(6)
UseMethanomethylophilus alvusTagsExpressionMutationCTG125CTT, N166A, V168S, A223C, W239RPromoterGlnS and LppAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRF0G-Tyr
Plasmid#113644PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.DepositorInsertMjYRS and tRNA gene
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pALS3-Ma-SUMO-sfGFP-WT
Plasmid#212122PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
UseTagsHis6 and SUMOExpressionBacterialMutationPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK-Ma pylRS nitroY/haloY-F5
Plasmid#212125PurposeExpresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS 3-NY/HaloY F5
UseTagsExpressionBacterialMutationCTG125CTT, N166A V168S, A223C, W239RPromoterGlnS (constitutive)Available sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKPY514
Plasmid#62598PurposeEncodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) ControlDepositorInsertThr251Gly-EcPheRS
UseTags6xHisExpressionBacterialMutationThr251GlyPromoterT5Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+NtEUt
Plasmid#170883PurposePhytobrick - NtEUtDepositorInsertNtEUt
UseTagsExpressionPlantMutationPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOSV00157
Plasmid#222937PurposeKill switch plasmid for DNA sensor construction using Bacillus subtilisDepositorInsertTxpA-RatA toxin-antitoxin
UseTagsExpressionBacterialMutationPromoterPhyperspankAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMC-5-Esp3I
Plasmid#197836PurposeLevel 1 module 5 with Esp3I expansion pointDepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-3-Esp3I
Plasmid#197832PurposeLevel 1 module 3 with Esp3I expansion pointDepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-3-BsaI
Plasmid#197820PurposeLevel 1 module 3 with BsaI expansion pointDepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-5-BsaI
Plasmid#197824PurposeLevel 1 module 5 with BsaI expansion pointDepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-1-BsaI
Plasmid#197816PurposeLevel 1 module 1 with BsaI expansion pointDepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-2T-BsaI
Plasmid#197817PurposeLevel 1 module 2T with BsaI expansion pointDepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-2-BsaI
Plasmid#197818PurposeLevel 1 module 2 with BsaI expansion pointDepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-3T-BsaI
Plasmid#197819PurposeLevel 1 module 3T with BsaI expansion pointDepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMC-4T-BsaI
Plasmid#197821PurposeLevel 1 module 4T with BsaI expansion pointDepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only