-
Plasmid#207098PurposepX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.DepositorInsertTTTTTATCAGAAATCATGAG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
HaloTag KO sgRNA
Plasmid#207102PurposepX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.DepositorInsertGTCGATGTTGGTCCGCGCGA
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MDC1 N-terminal sgRNA
Plasmid#207090PurposepX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.DepositorInsertGTATCCTTCCCAGATCATGG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHLD3 KO sgRNA #2
Plasmid#207106PurposepX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..DepositorInsertCTGAAGGAACAGACTAATTC
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
53BP1 KO sgRNA
Plasmid#207104PurposepX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.DepositorInsertAGATTCTCAGCCTGAAAGCC
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL8N_RB610-HBB_X1
Plasmid#191521PurposeExpression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAC, Right)DepositorInsertZF-DdCBE RB610-HBB X1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0597
Plasmid#196166PurposeMoClo Level 1 luciferase reporter: MinSyn_301 reporter [MinSyn_301::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_301 reporter [MinSyn_301::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0602
Plasmid#196168PurposeMoClo Level 1 luciferase reporter: MinSyn_303 reporter [MinSyn_303::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_303 reporter [MinSyn_303::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0601
Plasmid#196167PurposeMoClo Level 1 luciferase reporter: MinSyn_302 reporter [MinSyn_302::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_302 reporter [MinSyn_302::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0604
Plasmid#196169PurposeMoClo Level 1 luciferase reporter: MinSyn_305 reporter [MinSyn_305::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_305 reporter [MinSyn_305::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0607
Plasmid#196171PurposeMoClo Level 1 luciferase reporter: MinSyn_310 reporter [MinSyn_308::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_310 reporter [MinSyn_308::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0608
Plasmid#196172PurposeMoClo Level 1 luciferase reporter: MinSyn_311 reporter [MinSyn_308::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_311 reporter [MinSyn_308::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPYC1CB0595
Plasmid#196170PurposeMoClo Level 1 luciferase reporter: MinSyn_308 reporter [MinSyn_308::TMV::LucF::flag::ocsT]DepositorInsertMinSyn_308 reporter [MinSyn_308::TMV::LucF::flag::ocsT]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPSW1KN0035
Plasmid#196177PurposeMoClo Level 1 luciferase reporter: NOS::TMV::LucN::flag::nosTDepositorInsertNOS::TMV::LucN::flag::nosT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPSW1KN0018
Plasmid#196176PurposeMoClo Level 1 luciferase reporter: proARF18::LucN::flag::notTDepositorInsertproARF18::LucN::flag::notT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPSW1KN0020
Plasmid#196175PurposeMoClo Level 1 luciferase reporter: proNLP6::LucN::flag::notTDepositorInsertproNLP6::LucN::flag::notT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPSW1KN0014
Plasmid#196174PurposeMoClo Level 1 luciferase reporter: proANAC032::LucF::flag::notTDepositorInsertproANAC032::LucF::flag::notT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL5N_LT32-HBB_X1
Plasmid#191520PurposeExpression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAN, Left)DepositorInsertZF-DdCBE LT32-HBB X1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.004
Plasmid#184969PurposeNegative control, empty integrating inducible casetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p09008_AF1-4+GFP5-7
Plasmid#173715PurposePlasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.DepositorInsertshybrid GFP
mKate2
UseE. coli cell free protein synthesis extractsTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
BSP609
Plasmid#122254Purposeexpression vector for mTFP under EFT3 promoterDepositorInsertmTFP
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDHJS3 AN+
Plasmid#78240PurposeOne of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).DepositorInsertsloxP
sequence "A" with NheI site
loxP2
UsePhagemidTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS3 BS-
Plasmid#78239PurposeOne of four plasmids needed to create the double Holliday junction substrate (DHJS).DepositorInsertsloxP
sequence "A"
loxP2
UsePhagemidTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS3 AS+
Plasmid#78236PurposeOne of four plasmids needed to create the double Holliday junction substrate (DHJS).DepositorInsertsloxP
sequence "A"
loxP2
UsePhagemidTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDHJS3 BS+
Plasmid#78252PurposeOne of four plasmids needed to create the double Holliday junction substrate (DHJS).DepositorInsertsloxP
sequence "B"
loxP2
UsePhagemidTagsExpressionMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVD432
Plasmid#63755PurposeProbe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.DepositorInsertEAggEC
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD03 S. bayanus telomerase RNA KBS
Plasmid#61892PurposePlasmid for run-off in vitro transcription of Saccharomyces bayanus KBS RNADepositorInsertTelomerase RNA hairpin
UseTagsExpressionMutationPromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD05 S. castellii telomerase RNA KBS
Plasmid#61894PurposePlasmid for run-off in vitro transcription of Saccharomyces castellii KBS RNADepositorInsertTelomerase RNA hairpin
UseTagsExpressionMutationPromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD02 S. paradoxus telomerase RNA KBS
Plasmid#61891PurposePlasmid for run-off in vitro transcription of Saccharomyces paradoxus KBS RNADepositorInsertTelomerase RNA hairpin
UseTagsExpressionMutationPromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD04 S. arboricola telomerase RNA KBS
Plasmid#61893PurposePlasmid for run-off in vitro transcription of Saccharomyces arboricola KBS RNADepositorInsertTelomerase RNA hairpin
UseTagsExpressionMutationPromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCC1-4k-BBclone2
Plasmid#137070PurposeE. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1DepositorInsertsBB0323
P13
DipA
Lmp1
UseTagsExpressionBacterialMutationwild-type, full-length (signal-peptide-containing…PromoterAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSc1-DD
Plasmid#80439PurposeExpresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsE. coli dihydrofolate reductaseExpressionMammalianMutationPromoterCMV and U6Available sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
JARID1BA-c539
Plasmid#79643PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJARID1B (KDM5B Human)
UseBaculovirus expressionTags6x His and TEV cleavage siteExpressionMutationPromoterpolyhedrinAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Vimentin/pAS2-1
Plasmid#98136PurposeVimentin (NM_003380) in DNA-binding domain (BD) vector, used for yeast-two hybridDepositorInsertVimentin (VIM Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro
Plasmid#50417PurposeFluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.DepositorInsertseGFP with I-Sce I TS
+3 mCherry
UseLentiviralTagsT2A dislinker and iRFPExpressionMammalianMutationembedded I-Sce I TS from 163-185PromoterSFFVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28GSTlinker-LIC
Plasmid#119970PurposeStructure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Pinducer20-BSD-scFv-sfGFP-DNMT3A1
Plasmid#115780Purposetargeted DNA methylation toolDepositorInsertscFv-sfGFP-DNMT3A1 (DNMT3A Human)
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH
Plasmid#112211PurposeTargeted DNA methylationDepositorInsertC-terminus of dCas9
UseAAVTagsExpressionMutationD10APromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
prCOrev
Plasmid#153961PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
prCO
Plasmid#153960PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only