We narrowed to 30,799 results for: Eng
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Plasmid#129686Purpose(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoterDepositorInsertEmpty Backbone
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWDY1226
Plasmid#138593PurposeExpresses NatL2-R156A in E.coliDepositorInsertphenylacetate-coenzyme A ligase
UseTags6-HisExpressionBacterialMutationchanged Arginine 156 to AlaninePromoterT7Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWDY1222
Plasmid#138592PurposeExpresses NatL2-K418A in E.coliDepositorInsertphenylacetate-coenzyme A ligase
UseTags6-HisExpressionBacterialMutationchanged Lysine 418 to AlaninePromoterT7Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Bh
Plasmid#136378PurposeGateway entry clone for BhCas12b sgRNA expression under ZmUbi promoter with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Ace1_Q89D_D100N_E206V-HaloTag
Plasmid#129255PurposeExpress Ace1_Q89D_D100N_E206V-HaloTag in mammalian cellsDepositorInsertAce1_Q89D_D100N_E206V-HaloTag
UseTagsExpressionMammalianMutationQ89D, D100N, E206VPromoterCAGAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Mac_Q139D_D150N_E258V-HaloTag-ST
Plasmid#129264PurposeExpress soma-localized Mac_Q139D_D150N_E258V-HaloTag in mammalian cellsDepositorInsertMac_Q139D_D150N_E258V-HaloTag-ST
UseTagsExpressionMammalianMutationQ139D,D150N,E258VPromoterCAGAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag-ST
Plasmid#129263PurposeExpress soma-localized QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cellsDepositorInsertQuaAr3_Q95D_H106N_E214V-HaloTag-ST
UseTagsExpressionMammalianMutationQ95D, H106N, E214VPromoterCAGAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST
Plasmid#129260PurposeExpress soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cellsDepositorInsertAce1_Q89D_D100N_E206V-HaloTag-ST
UseTagsExpressionMammalianMutationQ89D, D100N, E206VPromoterCAGAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Mac_Q139D_D150N_E258V-HaloTag
Plasmid#129259PurposeExpress Mac_Q139D_D150N_E258V-HaloTag in mammalian cellsDepositorInsertMac_Q139D_D150N_E258V-HaloTag
UseTagsExpressionMammalianMutationQ139D,D150N,E258VPromoterCAGAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag
Plasmid#129258PurposeExpress QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cellsDepositorInsertQuaAr3_Q95D_H106N_E214V-HaloTag
UseTagsExpressionMammalianMutationQ95D, H106N, E214VPromoterCAGAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/68
Plasmid#113921PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68DepositorInsertMBP-NanoLucCP-65/68
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/66
Plasmid#113920PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66DepositorInsertMBP-NanoLucCP-65/66
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-LUCIDRS-MTX
Plasmid#113925PurposePlasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic biosensor for methotrexateDepositorInsertLUCID_RS-MTX
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7T3D-PacI-Tmeff2
Plasmid#99728Purposeto make in situ RNA probes for Tmeff2DepositorInsertTmeff2 (Tmeff2 Mouse)
UseIn situ probeTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
POT5-TDH3p-ycrtYB-ADH1t
Plasmid#65358PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTDH3p-ycrtYB-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT2-TDH3p-ycrtE-ADH1t
Plasmid#65352PurposeTU for combinatorial construction of ?-carotene pathwayDepositorInsertTDH3p-ycrtE-ADH1t
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_PLCG1:1-1291
Plasmid#211089PurposeMammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.DepositorInsertPLCG1:M1-L1291 (PLCG1 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationisoform 2. I813T natural variant (VAR_011908)PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_FBXW7:1-707
Plasmid#211099PurposeMammalian expression of full-length human FBXW7. N-terminal HiBiT tag.DepositorInsertFBXW7:M1-K707 (FBXW7 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_FBXW7:1-707
Plasmid#211104PurposeMammalian expression of full-length human FBXW7. C-terminal HiBiT tag.DepositorInsertFBXW7:M1-K707 (FBXW7 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-N_CCT5:1-541
Plasmid#210978PurposeMammalian expression of full-length human CCT5. N-terminal NanoLuc luciferase tag.DepositorInsertCCT5:M1-E541 (CCT5 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-N_FBXW7:1-707
Plasmid#210946PurposeMammalian expression of full-length human FBXW7. N-terminal NanoLuc luciferase tag.DepositorInsertFBXW7:M1-K707 (FBXW7 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC_DDB1:1-1140_delta:396-689
Plasmid#210897PurposeInsect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.DepositorInsertDDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
UseTagsExpressionInsectMutationloop deletion amino acids I396 to D689PromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNLF1-C_FBXW7:1-707
Plasmid#210951PurposeMammalian expression of full-length human FBXW7. C-terminal NanoLuc luciferase tag.DepositorInsertFBXW7:M1-K707 (FBXW7 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-avi-TEV-LIC_DCAF12:1-453
Plasmid#210940PurposeInsect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.DepositorInsertDCAF12:M1-S453 (DCAF12 Human)
UseTagsExpressionInsectMutationR131Q natural variant (VAR_035322)PromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_EIF3I:1-325
Plasmid#210910PurposeInsect expression of full-length human EIF3I. N-terminal 6X His tag with TEV cleavage.DepositorInsertEIF3I:M1-A325 (EIF3I Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBiT3.1-N_DDB1:1-1140
Plasmid#211147PurposeMammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. N-terminal HiBiT tag.DepositorInsertDDB1:M1-H1140 (DDB1 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationloop deletion I396-D689PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_DDB1:1-1140
Plasmid#211188PurposeMammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. C-terminal HiBiT tag.DepositorInsertDDB1:M1-H1140 (DDB1 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationloop deletion I396-D689PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_CCT5:1-541
Plasmid#210979PurposeMammalian expression of full-length human CCT5. C-terminal NanoLuc luciferase tag.DepositorInsertCCT5:M1-E541 (CCT5 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)
Plasmid#71814PurposeExpresses high specificity SpCas9. Px330-like plasmid.DepositorHas ServiceCloning Grade DNAInsertenhanced specificity Cas9 (1.1)
UseCRISPRTags3xFLAGExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF.hICN1.CMV.GFP
Plasmid#17623PurposeDepositorInsertICN1 (NOTCH1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-F-RAM-d2tTA-TRE-mKate2
Plasmid#140274PurposeF-RAM, the Fos-dependent reporterDepositorInsertmKate2
UseAAVTagsExpressionMutationPromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-N-RAM-d2tTA-TRE-mKate2
Plasmid#140275PurposeN-RAM, the Npas4-dependent reporterDepositorInsertmKate2
UseAAVTagsExpressionMutationPromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYL-kasOp-FnCas12a2
Plasmid#177874PurposeExpresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.DepositorInsertStreptomyces codon optimized FnCas12a
UseCRISPRTagsExpressionMutationPromoterkasOp*Available sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYL-kasOp-FnCas12a3
Plasmid#177875PurposeExpresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.DepositorInsertStreptomyces codon optimized the FnCas12a mutant EP16
UseCRISPRTagsExpressionMutationPromoterkasOp*Available sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA
Plasmid#176270PurposeExpression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cellsDepositorInsertTadA-TadA*(8e)-dCasMINI-V4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-11b_PcrA_M6_H93A
Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
UseTags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.(Cyto)iGlucoSnFR
Plasmid#164513PurposeGreen glucose sensor, cytosolicDepositorInsert(Cyto)iGlucoSnFR
UseAAVTagsExpressionMutationSynthetic ConstructPromoterGFAPAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
IVT-R2Tg_opt
Plasmid#223247PurposeIn vitro transcription of the optimized R2Tg proteinDepositorInsertR2Tg protein_opt
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2
Plasmid#164514PurposeGreen glucose sensor with mRuby2 fusion, cytosolicDepositorInsert(Cyto)iGlucoSnFR-mRuby2
UseAAVTagsExpressionMutationSynthetic ConstructPromoterGFAPAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-INPP5K
Plasmid#171950PurposeExpresses INPP5K in mammalian cellsDepositorInsertINPP5K (INPP5K Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA984
Plasmid#120363PurposeExpresses an single chain antibody 1C19 that targets all four DENV serotypes and expresses a red fluorescent protein reporterDepositorInsert1C19 single chain antibody
UseUnspecifiedTagsExpressionMutationPromoterAAEL010782 carboxypeptidase A (CPA) gene PromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-mito-Dendra2
Plasmid#107590PurposeExpresses mitochondria localized Dendra2 in zebrafish neutrophilsDepositorInsertmito-Dendra2
UseTagsmitochondira localization sequenceExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-polg guide
Plasmid#107598PurposeAn entry vector with U6a promoter driving polg guide RNA expressionDepositorInsertpolg guide RNA sequence
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-H-Luc_nucl
Plasmid#113931PurposePlasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cellsDepositorInsertH-Luc_nucl
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-H-Luc_cyto
Plasmid#113929PurposePlasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cellsDepositorInsertH-Luc
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-INPP5B
Plasmid#171947PurposeExpresses INPP5B in mammalian cellsDepositorInsertINPP5B (INPP5B Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGY53
Plasmid#221709PurposeEmpty Cas12a vector for multiplex gene editing in fungiDepositorTypeEmpty backboneUseCRISPR; AspergillusTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
IVT-nCas9(H840A)
Plasmid#207455Purposein vitro transcription of mRNADepositorInsertH840A
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DENV2-NS2B3_V5
Plasmid#115906PurposeMammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tagDepositorInsertDENV2 NS2B3 protease
UseTagsV5 tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-flex-Positron-ST
Plasmid#129267PurposeCre-dependant expression of soma-localized Positron in neuronsDepositorInsertPositron-ST
UseAAVTagsExpressionMammalianMutationD92N, E199VPromoterhsynAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only