Skip to main content
Addgene
Showing: 2401 - 2450 of 4070 results

  1. pHES830

    Plasmid
    #87944
    Purpose
    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.
    Depositor
    Hana El-Samad
    Article
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    Zif268 DBD - hPR LBD - MSN2 AD (PGR Human, Synthetic, Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 (crippled)
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    87944-plasmid-map-sequence-id-180365

    Enlarge

  2. pHES941

    Plasmid
    #87942
    Purpose
    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.
    Depositor
    Hana El-Samad
    Article
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    GAL4 DBD - hPR LBD - MSN2 AD (PGR Human, Synthetic, Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    87942-plasmid-map-sequence-id-180361

    Enlarge

  3. pHES873

    Plasmid
    #87945
    Purpose
    Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.
    Depositor
    Hana El-Samad
    Article
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    pGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Human, Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87945-plasmid-map-sequence-id-180366

    Enlarge

  5. pMK110

    Plasmid
    #99535
    Purpose
    contains bgl operon sequence for E. coli in vitro transcription template
    Depositor
    Robert Landick
    Article
    Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife
    Insert
    E. coli bgl antisense region containing bglG
    Use
    Tags
    None
    Expression
    Mutation
    Promoter
    lambda Pr promoter used for in vitro transcriptio…
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    99535-plasmid-map-sequence-id-190923

    Enlarge

  16. pcDNA3-PRB

    Plasmid
    #89130
    Purpose
    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418
    Depositor
    Elizabeth Wilson
    Article
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Insert
    human progesterone receptor-B (PGR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89130-plasmid-map-sequence-id-176077

    Enlarge

  17. p5M-hPR-B

    Plasmid
    #89114
    Purpose
    mammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinker
    Depositor
    Elizabeth Wilson
    Article
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Insert
    human progesterone receptor-B (PGR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5 restriction sites deleted in polylinker
    Promoter
    CMV
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    89114-plasmid-map-sequence-id-267772

    Enlarge

  19. pNB_EA_R1CiM_4EGFP_R3EGFPi2

    Plasmid
    #185968
    Purpose
    Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.
    Depositor
    Sangram Bagh
    Article
    Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-
    Insert
    Molecular IMPLY gate
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    185968-plasmid-map-sequence-id-370628

    Enlarge

  21. C0062 (luxR)_CD

    Plasmid
    #66028
    Purpose
    MoClo Basic Part: CDS - Controller protein, luxR repressor/activator (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates pLuxR(pL) R0062) [C:C0062:D]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Transcription factor
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66028-plasmid-map-sequence-id-114215

    Enlarge

  22. pLPT234

    Plasmid
    #127855
    Purpose
    Repressilator 2.0, without degradation tags, integrated triple reporter
    Depositor
    Johan Paulsson
    Article
    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:
    Inserts
    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    First 11aa derived from mCherry as fusion protein
    Promoter
    pAmpR, pL LacO1, pL tetO1, and pR
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    127855-plasmid-map-sequence-id-253039

    Enlarge

  39. TFORF2960

    Plasmid
    #144436
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PGR (PGR Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144436-plasmid-map-sequence-id-407325

    Enlarge

  40. TFORF1691

    Plasmid
    #142827
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PGR (PGR Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142827-plasmid-map-sequence-id-396021

    Enlarge

  49. 8V04

    Plasmid
    #220879
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS2 (TMPRSS2 Human)
    Use
    Baculovirus expresssion
    Tags
    EFVEHHHHHHHH and Honeybee melittin signal
    Expression
    Mutation
    S251D/R252D/Q253D/S254D/R255K
    Promoter
    Polyhedrin
    Available since
    Sept. 30, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220879-plasmid-map-sequence-id-436702

    Enlarge

  50. 8VIS

    Plasmid
    #220881
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS11D (TMPRSS11D Human)
    Use
    Baculovirus expresssion
    Tags
    EFVEHHHHHHHH
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    Sept. 30, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220881-plasmid-map-sequence-id-436700

    Enlarge

Showing: 2401 - 2450 of 4070 results