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Showing: 2401 - 2450 of 17291 results

  4. pPEE47

    Plasmid
    #139426
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mNeonGreen
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    139426-plasmid-map-sequence-id-297721

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  5. pPEE46

    Plasmid
    #139425
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mRuby3
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    139425-plasmid-map-sequence-id-292473

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  10. pRN3P-NLS-EGFP-deadBtgh-3NLS

    Plasmid
    #194470
    Purpose
    Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Article
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Mutation
    Aspartic Acid 242 (in Dennis et al.) changed to A…
    Available Since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    194470-plasmid-map-sequence-id-384877

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  13. pDGB1alpha11_SulR

    Plasmid
    #186424
    Purpose
    transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assembly
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Insert
    SulR
    Use
    Synthetic Biology; Binary vector for escherichia …
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186424-plasmid-map-sequence-id-369067

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  35. pTF101.1gw3

    Plasmid
    #172181
    Purpose
    A binary plasmid for gateway 3-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.
    Depositor
    Kan Wang
    Article
    Paz et al Plant Cell Rep. 2006 Mar;25(3):206-13. d
    Insert
    attR3-CmR-ccdB-attR4
    Expression
    Plant
    Promoter
    lacUV5 promoter
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    172181-plasmid-map-sequence-id-343829

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  36. pPEE42

    Plasmid
    #139421
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    Citrine
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    139421-plasmid-map-sequence-id-324126

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  37. pPEE45

    Plasmid
    #139424
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mTurquoise1
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139424-plasmid-map-sequence-id-313811

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  38. pPEE44

    Plasmid
    #139423
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mTFP1
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139423-plasmid-map-sequence-id-313810

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  39. pPEE43

    Plasmid
    #139422
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mMaroon1
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139422-plasmid-map-sequence-id-313808

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  42. pCIG1_UL136_19kDa_EE

    Plasmid
    #74951
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Glu-Glu tag (EYMPME)
    Expression
    Mammalian
    Mutation
    delta 1-127
    Promoter
    CMV, lentiviral LTR
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    74951-plasmid-map-sequence-id-218061

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  43. pCIG1_UL136_23kDa_Myc

    Plasmid
    #74949
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    HA Tag (YPYDVPDYA)
    Expression
    Mammalian
    Mutation
    delta 1-99 M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    74949-plasmid-map-sequence-id-218062

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  44. pCIG1_UL136_25kDa_Myc

    Plasmid
    #74954
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)
    Expression
    Mammalian
    Mutation
    delta 1-77, M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74954-plasmid-map-sequence-id-141774

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  47. GST-mDia1(FH1FH2DAD)-His

    Plasmid
    #85822
    Purpose
    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.
    Depositor
    Antoine Jégou, Guillaume Romet-Lemonne
    Article
    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc
    Insert
    Protein diaphanous homolog 1 (Diaph1 Mouse)
    Tags
    8*His and GST
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85822-plasmid-map-sequence-id-183287

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  48. FB007

    Plasmid
    #119705
    Purpose
    Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter gpdA
    Use
    Synthetic Biology; Domestication of dna parts for…
    Promoter
    gpdA
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119705-plasmid-map-sequence-id-237147

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  49. Rlucrarr3-Flash5

    Plasmid
    #74133
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Available Since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  50. Rlucrarr3-Flash6

    Plasmid
    #74134
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Available Since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2401 - 2450 of 17291 results