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Plasmid#236491PurposePlasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Nat.DepositorInsertmScarlet
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-sfGFP-G418
Plasmid#236481PurposePlasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on G418.DepositorInsertsfGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mCherry-Hyg
Plasmid#236489PurposePlasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Hyg.DepositorInsertmCherry
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-Dendra2-G418
Plasmid#236496PurposePlasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on G418.DepositorInsertDendra2
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-GFP-Nat
Plasmid#236476PurposePlasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Nat.DepositorInsertGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-Dendra2-Hyg
Plasmid#236495PurposePlasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Hyg.DepositorInsertDendra2
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mNeonGreen-Hyg
Plasmid#236483PurposePlasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Hyg.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mScarlet-G418
Plasmid#236493PurposePlasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on G418.DepositorInsertmScarlet
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mScarlet-Hyg
Plasmid#236492PurposePlasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Hyg.DepositorInsertmScarlet
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-GFP-Hyg
Plasmid#236477PurposePlasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Hyg.DepositorInsertGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-sfGFP-Nat
Plasmid#236479PurposePlasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Nat.DepositorInsertsfGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mNeonGreen-Nat
Plasmid#236482PurposePlasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-GFP-G418
Plasmid#236478PurposePlasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.DepositorInsertGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST
Plasmid#213143PurposeTtgV BiosensorDepositorInsertTtgV sfGFP Biosensor
UseTagsExpressionBacterialMutationPromoterTacAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSR1154
Plasmid#221653PurposeTetracycline sensor v3 for Staphylococcus aureusDepositorInsertssfGFP cassette
TetR cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPXyl-TetO and PZwfAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCF
Plasmid#216326PurposePUlaA promoter driving expression of sfgfpDepositorInsertSuperfolder green fluorescent protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUlaR
Plasmid#216327PurposeT7 promoter driving expression of ulaRDepositorInsertL-Ascorbate-6-P-responsive transciptional repressor
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBad_UlaABC
Plasmid#216328PurposeArabinose-inducible PBad promoter driving expression of ulaA, ulaB, and ulaCDepositorInsertUlaABC
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-6xHis-GFP(-7)-neg_tag1
Plasmid#219419PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertGFP(-7)-neg_tag1
UseTagsDEEEDD and MGHHHHHGGExpressionBacterialMutationPromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJEC637
Plasmid#182956PurposeAD-SYNZIP: αNTD (P. aeruginosa) – SYNZIP17DepositorInsertAlphaNTD( P. aeruginosa) - SynZip17
UseTagsSynZip17ExpressionBacterialMutationPromoterJ23106Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only