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pU6-cGAS g2 Cas9-T2A-mCherry

Plasmid

#164253

Purpose

Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgcGAS

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCyPet-C1

Plasmid

#22779

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

CyPet

Expression

Mammalian

Mutation

Control for intermolecular Rac1 biosensor FRET. S…

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only
pYpet-C1

Plasmid

#22780

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Ypet

Expression

Mammalian

Mutation

Control for intermolecular Rac1 biosensor FRET. S…

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only
pBEL2511

Plasmid

#195625

Purpose

Complementation of MlaA(WT)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-Rsn2

Plasmid

#61686

Purpose

bacterial expression of ranaspumin 2 as a His-tagged fusion protein

Depositor

Brian Smith

Article

Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

Insert

Ranaspumin-2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-cGAS g3 Cas9-T2A-mCherry

Plasmid

#164254

Purpose

Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgcGAS

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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VV020: WT Arch-3 - eGFP in pET28b

Plasmid

#58488

Purpose

Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

Depositor

Adam Cohen

Article

Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Archaerhodopsin-3

Tags

eGFP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pOC4 cloning template vector

Plasmid

#183423

Purpose

FlpON knock-in vector with FLAG-SpCas9 expression

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDU1

Plasmid

#169817

Purpose

pSpCas9(BB)-2A-tCD19 (pDU1)

Depositor

Andrea Rossi

Article

Ramachandran et al Life Sci Alliance. 2021 Apr 26;4(6). pii

Insert

tCD19

Tags

fusion protein

Expression

Mammalian

Promoter

Cbh

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-scFv-VP64_Blast

Plasmid

#192655

Purpose

3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

scFv-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-TREX1 g2 Cas9-T2A-mCherry

Plasmid

#164251

Purpose

Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgTREX1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pPM2212

Plasmid

#167552

Purpose

Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

Depositor

Renato Morona

Article

Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

Insert

rfb region of Shigella flexneri serotype 2a

Use

Cosmid

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPL5628_TEF1*-Cre_MET15

Plasmid

#60933

Purpose

Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Promoter

mutant TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-TREX1 g1 Cas9-T2A-mCherry

Plasmid

#164250

Purpose

Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgTREX1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pQUAST-syb-spCFP1-10

Plasmid

#73699

Purpose

Neuronal-synaptobrevin (syb):spCFP1–10 chimera for synaptic GRASP

Depositor

Marco Gallio

Article

Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

Insert

Neuronal-synaptobrevin:spCFP1–10 chimera

Expression

Insect

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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WT Nedd4L

Plasmid

#200086

Purpose

Nedd4L in pQCXIN vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

Nedd4L (NEDD4L Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pF3-CAG-Tol-FRT

Plasmid

#141349

Purpose

Shuttling cassette containing the Tol ORF

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

Tol cassette

Use

Mouse Targeting

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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knob-s48i48

Plasmid

#68391

Purpose

Knob protein fused to elastin-like polypeptide (S48I48)

Depositor

Andrew MacKay

Article

Sun et al J Control Release. 2011 Oct 30;155(2):21

Insert

Knob-S48I48

Tags

Knob

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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p44PPV-eGFP

Plasmid

#241907

Purpose

Part 2 of a two-plasmid PCA biosensor composed of the PcaV repressible PPV promoter upstream from a reporter gene (eGFP) on a second plasmid

Depositor

Neil Dixon

Article

F M Machado et al J Biol Eng. 2019 Nov 27;13:91. doi: 10.1

Insert

His6-eGFP

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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DriD-wHTH-DNABD

Plasmid

#237851

Purpose

The wHTH DNA binding domain used to understand the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD wHTH DNABD

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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