We narrowed to 1,302 results for: SUMO

Showing: 961 - 980 of 1302 results

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pSMT3-FBF2

Plasmid

#166972

Purpose

Expresses FBF-2 in E. coli for purification and crystallization studies

Depositor

Traci Hall

Article

Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

Insert

FBF-2 (fbf-2 Nematode)

Tags

His-SUMO

Expression

Bacterial

Mutation

FBF-2 PUM RNA binding domain; amino acids 164-575

Promoter

T7

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-C93A

Plasmid

#133910

Purpose

Bacterial expression of His6-Ubc9-C93A

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 93 to Alanine

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-C138A

Plasmid

#133911

Purpose

Bacterial expression of His6-Ubc9-C138A

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 138 to Alanine

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCA528 CAPN7 (1-75)

Plasmid

#193031

Purpose

Bacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domain

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

Residues 1-75

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CAPN7 (81-165)

Plasmid

#193032

Purpose

Bacterial expression plasmid for CAPN7 (81-165) C-terminal MIT domain

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

Residues 81-165

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP7 366-453

Plasmid

#104598

Purpose

Expresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP7 residues 366-453 (CHMP7 Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTVKRSF225

Plasmid

#135005

Purpose

MusF2 prenyltransferases from Nmu. PCC 7906

Depositor

Hideo Iwai

Article

Mattila et al ACS Chem Biol. 2019 Dec 20;14(12):2683-2

Insert

MusF2 prenyltransferaseM

Tags

SUMO fusion

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBHRSF227

Plasmid

#135006

Purpose

MusF1 prenyltransferase from Nostoc sp. UHCC 0398

Depositor

Hideo Iwai

Article

Mattila et al ACS Chem Biol. 2019 Dec 20;14(12):2683-2

Insert

MusF1 prenyltransferase

Tags

SUMO fusion

Expression

Bacterial

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

Plasmid

#199604

Purpose

Expression of cell permeable spCas9 in E. Coli.

Depositor

Junwei Shi

Article

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

Cas9-T6N

Use

CRISPR

Expression

Bacterial

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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WDV-Echistatin

Plasmid

#226985

Purpose

Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

Depositor

Wendy Gordon

Article

Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

Inserts

WDV (repA )
Echistatin

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF-rM1PYK

Plasmid

#220232

Purpose

Inducible expression of rabbit muscle pyruvate kinase isoform 1

Depositor

Aron Fenton, Liskin Swint-Kruse

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Rabbit muscle pyruvate kinase (PKM )

Tags

6xHis-MBP-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHTSHP-Smt-IIShhN

Plasmid

#121135

Purpose

Expresses N-terminal SUMO tagged II-ShhN in bacteria

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Shh (Shh Mouse)

Tags

HIS, HRV3C site, MBP, and Smt tag

Expression

Bacterial

Mutation

Amino acid sequence from 26-195, with two extra i…

Promoter

tac

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCA528 huPQBP1

Plasmid

#217449

Purpose

Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

PQBP1 (PQBP1 Human)

Expression

Bacterial

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1 (322-366)

Plasmid

#193038

Purpose

Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

Residues 322-366

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1 L328D, L355A (1-366)

Plasmid

#193040

Purpose

Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

L328D, L355A

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CAPN7 L61D (1-165)

Plasmid

#193033

Purpose

Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

L61D, Residues 1-165

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CAPN7 V18D,F98D (1-165)

Plasmid

#193034

Purpose

Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

V18D, F98D, Residues 1-165

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1, residues 316-366, C-Cys

Plasmid

#191234

Purpose

Bacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labeling

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 316-366, contains nonnative C-terminus f…

Promoter

T7

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP4A 153-222

Plasmid

#108256

Purpose

Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP4A residues 153-222 (CHMP4A Human)

Tags

His-SUMO

Expression

Bacterial

Mutation

Contains nonnative Gly-Cys for fluorescent labeli…

Promoter

T7

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP4C 156-233

Plasmid

#108257

Purpose

Expresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP4C residues 156-233 (CHMP4C Human)

Tags

His-SUMO

Expression

Bacterial

Mutation

Contains nonnative Gly-Cys for fluorescent labeli…

Promoter

T7

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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