We narrowed to 2,461 results for: T2A

1. AAV-CAG-FLEX-NEUROD1-T2A-mCherry

   Plasmid

   #178584

   Purpose

   Cre-dependent expression of NEUROD1 and mCherry under the constitutive promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   NEUROD1 (NEUROD1 Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

   Plasmid

   #128166

   Purpose

   Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   mTagBFP2-T2A-sfGFP-iCre-ERT2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFH2.96_PB-EPN1-T2A-eGFP-PGK-Blast

   Plasmid

   #205541

   Purpose

   Express EPN1-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN1
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFH2.100_PB-EPN11-T2A-eGFP-PGK-Blast

   Plasmid

   #205545

   Purpose

   Express EPN11-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN11
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

   Plasmid

   #78307

   Purpose

   Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-nlsLexA::GADflluw
   

   Available Since

   July 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

   Plasmid

   #193915

   Purpose

   Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Farnesylation signal sequence and Flag tag

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRL-MND-hCard11 R72L-T2A-GFP

   Plasmid

   #163784

   Purpose

   Expression of R72L CARD domain variant

   Depositor

   Richard James

   Article

   Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

   Insert

   CARD11 (CARD11 Human)
   

   Use

   Lentiviral

   Mutation

   R72L

   Promoter

   MND

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1-mouse SmyD1-T2A-copGFP

   Plasmid

   #101157

   Purpose

   Lentiviral expression of SmyD1 and copGFP

   Depositor

   Martine Roussel

   Article

   Wei et al Oncotarget. 2017 Jul 27. doi: 10.18632/o

   Insert

   SmyD1 (Smyd1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-FNLS-T2A-EGFP-ires-puro

   Plasmid

   #121169

   Purpose

   CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

   Depositor

   Timo Otonkoski

   Insert

   FNLS-T2A-EGFP-ires-puro
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV hSyn1 bPAC cMyc T2A tDimer

    Plasmid

    #85397

    Purpose

    humanized photoactivated adenylyl cyclase with cMyc Tag driven by neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Inserts

    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    

    Use

    AAV

    Tags

    cMyc Tag

    Expression

    Mammalian

    Promoter

    human synapsin-1 and ribosomal skip sequence T2A

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta

    Plasmid

    #79913

    Purpose

    piggyBac transposon vector, Zeocin-2A-sHRPaNRX3beta

    Depositor

    Joshua Sanes

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Zeocin-t2A-sHRPbNRX3beta
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid

    #129048

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA8 (huTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid

    #129041

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid

    #129036

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA8 (huTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid

    #121171

    Purpose

    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    xFNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

    Plasmid

    #129053

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA1 (mouseTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1

    Plasmid

    #79914

    Purpose

    piggyBac transposon vector, Zeocin-2A-sHRPbNLG1

    Depositor

    Joshua Sanes

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Zeocin-t2A-splitHRP-aNLG1 (Nlgn1 Mouse)
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid

    #129029

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA1 (huTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CAG-loxP-3xpA-sfGFP-tdTomato T2A V XE

    Plasmid

    #113853

    Purpose

    Superfolder GFP and tdTomato linked by T2A (V linker)

    Depositor

    Mario Capecchi

    Insert

    sfGFP-T2A-tdTomato
    

    Expression

    Mammalian

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBW708_pCAG-PV4-loxP-GFP-T2A-mCherry

    Plasmid

    #89057

    Purpose

    PV4 for 2-input, 2-output circuit library

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    GFP-T2A-mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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