We narrowed to 1,270 results for: grna cloning vector

1. pDONR221 CARD8 PAM S297A

   Plasmid

   #169976

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   PAM, S297A

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221 CARD8 PAM F370G

   Plasmid

   #169979

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   PAM, F370G

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 CARD8 PAM F405G

   Plasmid

   #169981

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   PAM, F405G

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221 CARD8 PAM E274R

   Plasmid

   #169977

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   PAM, E274R

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIA123

   Plasmid

   #234038

   Purpose

   E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.

   Depositor

   Craig Ellermeier

   Article

   Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Pgdh and Pxyl

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIA33

   Plasmid

   #120803

   Purpose

   E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

   Depositor

   David S. Weiss, Craig Ellermeier

   Article

   Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

   Inserts

   deactivated nuclease dCas9, codon-optimized for C. difficile
   single guide RNA targeting red fluorescent protein
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Base-pairing region targets red fluorescent prote…

   Promoter

   Pgdh and Pxyl

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sa-SlugCas9

   Plasmid

   #163797

   Purpose

   Expresses Sa-SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SlugCas9-HF

   Plasmid

   #163796

   Purpose

   Expresses highly specific SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   SlugCas9 (R247A, N415A, T421A, R656A)

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRU41

   Plasmid

   #167663

   Purpose

   ENTRY vector for gRNA1 cloning under pAtU6 promoter

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pAtU6
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRU42

    Plasmid

    #167664

    Purpose

    ENTRY vector for gRNA2 cloning under pAtU3 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU3
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRU43

    Plasmid

    #167665

    Purpose

    ENTRY vector for gRNA3 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRU44

    Plasmid

    #167666

    Purpose

    ENTRY vector for gRNA4 cloning under pAtU3 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU3
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPE4max-Blasticidin

    Plasmid

    #194905

    Purpose

    Expressing prime editing PE4max proteins, to work in conjunction with an epegRNA encoding plasmid or Circular Vector as described in the cited article.

    Depositor

    George Church

    Article

    Oliynyk et al Commun Biol. 2022 Dec 21;5(1):1393. doi:

    Insert

    BSD
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRU45

    Plasmid

    #167667

    Purpose

    ENTRY vector for gRNA5 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRU46

    Plasmid

    #167668

    Purpose

    ENTRY vector for gRNA6 cloning under pAtU3 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU3
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRU48

    Plasmid

    #167670

    Purpose

    ENTRY vector for gRNA8 cloning under pAtU3 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU3
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRU47

    Plasmid

    #167669

    Purpose

    ENTRY vector for gRNA7 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSIREN-RetroQ-shN

    Plasmid

    #73665

    Purpose

    pSIREN-RetroQ vector containing control sequence N

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA Neg Control
    

    Use

    Retroviral

    Available Since

    March 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pDECKO_GFP

    Plasmid

    #141210

    Purpose

    empty GFP vector for cloning sgRNA or pgRNAs

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDYT002

    Plasmid

    #186550

    Purpose

    Lineage tracing vector (with barcoded Target Sites and triple sgRNAs

    Depositor

    Jonathan Weissman

    Article

    Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00

    Insert

    14-bp random integration barcode and three target sites
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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