We narrowed to 15,052 results for: pET

1. pETSUMO2_Vitiosangium-bGSDM

   Plasmid

   #212127

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Vitiosangium bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETSUMO2_Gillisia-bGSDM

   Plasmid

   #212128

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Gillisia bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETSUMO2_Bradyrhizobium-bGSDM

   Plasmid

   #212126

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Bradyrhizobium bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET47b-T7-dAsCas12f

   Plasmid

   #204641

   Purpose

   Expression of an inactive AsCas12f in E. coli

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   inactive AsCas12f
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D225A

   Promoter

   T7

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL1-CyPet

   Plasmid

   #102636

   Purpose

   In vitro expression of CyPet from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   CyPet
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-Kss1

   Plasmid

   #52682

   Purpose

   Expression of Kss1 in bacteria

   Depositor

   Benjamin Turk

   Article

   Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

   Insert

   KSS1 (KSS1 Budding Yeast)
   

   Tags

   His6 and T7 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pET23a-LIR-1 D1-D2

   Plasmid

   #12103

   Depositor

   Pamela Bjorkman

   Article

   Willcox et al Nat Immunol. 2003 Sep . 4(9):913-9.

   Insert

   Leukocyte immunoglobulin-like receptor 1 (LILRB1 Human)
   

   Expression

   Bacterial

   Mutation

   Amino acids 1−198*

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pET28a(+)-SUMO-A'-l4-G'

   Plasmid

   #209124

   Purpose

   Bacterial expression of CC-GEMS ligand SUMO-A'-l4-G'

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'-l4-G'
   

   Expression

   Bacterial

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. petRNA-FANCF-v3'

   Plasmid

   #181803

   Purpose

   petRNA FANCF v3'

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

   Insert

   ribozyme-petRNA
   

   Use

   CRISPR

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Grp1-PH pET11a

    Plasmid

    #71377

    Purpose

    Phosphoinositide Binding

    Depositor

    Mark Lemmon

    Article

    Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

    Insert

    Grp1-PH (Cyth3 Mouse)
    

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 264-391

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pETCON-TNF

    Plasmid

    #67748

    Purpose

    Tumor Necrosis Factor Alpha Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    TNF-Alpha
    

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET24a MDH_STRAW

    Plasmid

    #204240

    Purpose

    Bacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_STRAW (mdh Streptomyces avermitilis)
    

    Tags

    6X His

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-Tyr-P(11)4

    Plasmid

    #185784

    Purpose

    Encodes enzyme tyrosinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    Tyr-P(11)4
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM41-EcPPK

    Plasmid

    #38334

    Depositor

    Florian Freimoser

    Insert

    E. coli polyphosphate kinase (ppk Escherichia coli)
    

    Tags

    6xHIS, MBP, and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

    Plasmid

    #116965

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15-EF4n_mFAP2b

    Plasmid

    #159464

    Purpose

    Encodes for EF4n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF4n_mFAP2b
    

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21c-IF3mt

    Plasmid

    #31078

    Depositor

    Linda Spremulli

    Article

    Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

    Insert

    Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Includes coding sequence for the mature form. Ami…

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

18. M26I DJ-1 pET15b

    Plasmid

    #60677

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

    Insert

    M26I DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26I

    Promoter

    T7

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pET15-EF1p3_mFAP2b

    Plasmid

    #159470

    Purpose

    Encodes for EF1p3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF1p3_mFAP2b
    

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET15-mFAP10

    Plasmid

    #159463

    Purpose

    Encodes for mFAP10 with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    mFAP10
    

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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