We narrowed to 1,267 results for: piggyBac

1. pPB-TRE-ECC-Biosensor

   Plasmid

   #140833

   Purpose

   Encoding the TRE promoter based eccDNA biosensor

   Depositor

   Yonglun Luo

   Article

   Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

   Insert

   TRE-ECC Biosensor
   

   Use

   Piggybac (pb) transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   inverse

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

   Plasmid

   #154774

   Purpose

   plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   in PylT: UUA anticodon and G10A, G14U, G16U, C20U…

   Promoter

   EF1

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor

   Plasmid

   #184951

   Purpose

   Donor for both PiggyBac and Cp36 Recombinase

   Depositor

   Ami Bhatt, Michael Bassik, Lacra Bintu, Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   PuroR, P2A, mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

   Plasmid

   #84241

   Purpose

   Expresses ABA-inducible KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sp dCas9
   PYL1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-LacI-eGFP

   Plasmid

   #213021

   Purpose

   piggyBac donor vector containing LacI-eGFP for random integration by PBase

   Depositor

   Luca Giorgetti

   Article

   Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

   Insert

   LacI**-eGFP
   

   Use

   Mouse Targeting; Piggybase integration (transposo…

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-CAG::Cytbow

   Plasmid

   #158995

   Purpose

   Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

   Depositor

   Jean Livet

   Article

   Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

   Insert

   Cytbow
   

   Use

   Tags

   The default expressed EBFP2 is fused to H2B

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSH231-Bx-GFP-C31

   Plasmid

   #115149

   Purpose

   Empty safe harbor site 231 knock-in vector with flanking homology arms and Bxb1 and C31 attP integrase landing pads

   Depositor

   Michael Phelps, Raymond Monnat

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   Safe Harbor Homology Sequences
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

   Plasmid

   #198337

   Purpose

   PB plasmid expressing Halo-tagged PP7 coat protein

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   PP7 coat protein 3x Halo-GB1
   

   Use

   Piggybac

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

   Plasmid

   #140018

   Purpose

   Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   EcoTyrRS
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y37L, D182S, F183M, L186A

   Promoter

   EF1

   Available since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xMma PylT_FLAG-AcKRS

    Plasmid

    #140021

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    AcKRS-3 (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L301M, L305I, Y306F, L309A, C348F

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB[U6-3 wex2 PUb-DsRed]

    Plasmid

    #169010

    Purpose

    For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    white ex2 sgRNA
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster U6:3

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xG1 PylT FLAG-G1 PylRS Y125A

    Plasmid

    #154769

    Purpose

    plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y125A

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid

    #84240

    Purpose

    Expresses GA-inducible VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

    Plasmid

    #168107

    Purpose

    PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Insert

    Cas9-P2A-mCherry
    

    Use

    CRISPR and Cre/Lox

    Tags

    HA

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPBC-G6s

    Plasmid

    #62812

    Purpose

    pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-GCaMP6s-ITR
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBC-G6f

    Plasmid

    #62811

    Purpose

    pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-GCaMP6f-ITR
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. BII-ChiNW-IGF2

    Plasmid

    #133383

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    IGF2 (IGF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TetR-tdTomato

    Plasmid

    #212958

    Purpose

    piggyBac donor vector containing TetR-tdTomato for random integration by PBase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    TetR-tdTomato
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pB-Ric8b-Integration

    Plasmid

    #129457

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses Ric8b

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    Ric8b (Ric8b Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPBC-LG6s

    Plasmid

    #62809

    Purpose

    pPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-Lck-GCaMP6s-ITR
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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