We narrowed to 1,376 results for: piggyBac

1. PB-EF1-IRES-NEO deleted ITR

   Plasmid

   #67906

   Purpose

   PiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeat

   Depositor

   Alex Kentsis

   Article

   Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

   Insert

   NeoR (neoR Synthetic)
   

   Expression

   Mammalian

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC150-RFP-EGFP-LGALS3 R186S

   Plasmid

   #175779

   Purpose

   PiggyBac Vector expressing GFP-RFP-LGALS3 R186S

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   LGALS3 (LGALS3 Human)
   

   Expression

   Mammalian

   Mutation

   R186S

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSH231-EF1-Puro

   Plasmid

   #115143

   Purpose

   Safe harbor site 231 knock-in vector with puromycin resistance selection cassette

   Depositor

   Raymond Monnat, Michael Phelps

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   Puromycin
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB[nos-Cas9 PUb-ZsGreen]

   Plasmid

   #169011

   Purpose

   For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   Cas9
   

   Use

   Piggybac

   Expression

   Insect

   Promoter

   Drosophila melanogaster nanos

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

   Plasmid

   #102885

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-Blast

   Plasmid

   #205552

   Purpose

   Express EPN24-MCP-p6-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP-p6
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. BII-ChBtW-IGF2

   Plasmid

   #133374

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   IGF2 (IGF2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-CAG-BGHpA

   Plasmid

   #92161

   Purpose

   For lncRNA overexpression (in trans)

   Depositor

   Xiaohua Shen

   Article

   Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6c

   Plasmid

   #117223

   Purpose

   Expresses gRNA in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

   Insert

   HR U6c promoter-gRNA
   

   Use

   Synthetic Biology

   Expression

   Insect

   Promoter

   U6

   Available Since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6b

    Plasmid

    #117222

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    HR U6b promoter-gRNA
    

    Use

    Synthetic Biology

    Expression

    Insect

    Promoter

    U6

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. BII-ChBtW-ASCL2

    Plasmid

    #133375

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    ASCL2 (ASCL2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. BII-ChiNW-ASCL2

    Plasmid

    #133384

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    ASCL2 (ASCL2 Human)
    

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

    Plasmid

    #179586

    Purpose

    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    NLS-NG-T2A-hSHH
    

    Use

    Piggybac

    Tags

    NeonGreen

    Promoter

    TRE

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAEL006511-Cas9

    Plasmid

    #100580

    Purpose

    The promoter of AAEL006511 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL006511-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Promoter

    AAEL006511 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6c-T

    Plasmid

    #117211

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    u6c promoter-gRNA
    

    Use

    Synthetic Biology

    Expression

    Insect

    Promoter

    U6

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xMx PylT_FLAG-Mx PylRS YA

    Plasmid

    #140012

    Purpose

    Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y126A

    Promoter

    EF1

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-EF1-IRES-NEO mutant ITR

    Plasmid

    #67905

    Purpose

    PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

    Depositor

    Alex Kentsis

    Article

    Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

    Insert

    NeoR (neoR Synthetic)
    

    Expression

    Mammalian

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xMma PylT(UUA)_FLAG-AcKRS

    Plasmid

    #140010

    Purpose

    Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    AcKRS-3 (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L301M, L305I, Y306F, L309A, C348F

    Promoter

    EF1

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAEL007097-Cas9

    Plasmid

    #100705

    Purpose

    The promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL007097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Promoter

    AAEL007097 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BII-gR-BtW-eSpCas9

    Plasmid

    #133358

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Tags

    Flag-tagged eSpCas9

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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