We narrowed to 16,820 results for: RAN-1
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pEF1a-nSpCas9-NG
Plasmid#171692PurposeExpresses nSpCas9-NG in mammalian cellsDepositorInsertnSpCas9-NG
UseCRISPRExpressionMammalianMutationD10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…PromoterEF1aAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1.2-GS-eGFP
Plasmid#162771PurposeFluorescent reporter for CHO expression studiesDepositorInsertenhanced green fluorescent protein
ExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRha-tns
Plasmid#170630PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTnsABC
ExpressionBacterialAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_N-DYK_MTHFD1-NLS
Plasmid#133023PurposeNLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)DepositorAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTM
Plasmid#162783PurposeProtein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are codedDepositorTypeEmpty backboneTags10xHis, c-myc, and hTPA leaderExpressionMammalianPromoterCHO Elongation factor 1AAvailable SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-hgp100-PuroR [M1G]
Plasmid#171801PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-hgp100-T2A-PuroR
UseGene taggingMutationPuroR: Changed Methionin 1 to Glycine.Available SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO CPTP
Plasmid#170735PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SRGAP1FBAR(516a.a.)-EGFP
Plasmid#168505PurposeExpression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.DepositorAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS21: pHelper(PmcCAST)_entry
Plasmid#168154PurposeInducible expression of PmcCAST proteins. Two BsaI sites for spacer cloning.DepositorInsertsPmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS8: pTns(AvCAST)
Plasmid#168141PurposeInducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins.DepositorInsertsAvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS6: pHelper(AvCAST)_entry_ΔTnsD
Plasmid#168139PurposeInducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.DepositorInsertsAvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OK+9MS-Cas9
Plasmid#120354PurposepiggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.DepositorInsertOK+9MS cassette
UseCRISPR; Piggybac transposonExpressionMammalianMutationKlf4 [1-483]Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_CMV_HMGA_linker_mKate2
Plasmid#167335PurposeExpresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cellsDepositorInsertTFAM (TFAM Human)
UseLentiviralTagsmKate2ExpressionMammalianMutationTruncation mutant containing MTS (1-42) as well a…PromoterCMVAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-KIF5A-HA-IRES-Puro
Plasmid#166952PurposeLentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoterDepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
KLC1-Myc
Plasmid#166962PurposeExpresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1DepositorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-KIF5A tail only
Plasmid#166956PurposeExpresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1DepositorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-KIF5A
Plasmid#166958PurposeExpresses HA-tagged KIF5A protein in pcDNA3.1DepositorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
SnFR-gamma2
Plasmid#165495PurposeGreen fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.DepositorInsertiGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)
ExpressionMammalianPromoterCMVAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) CamKII sf iGluSnFR
Plasmid#158769PurposeiGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.DepositorInsertiGluSnFR
UseLentiviralMutationAdditional ER and Golgi export sequencesAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only
