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pUB-cSypHer

Plasmid

#84733

Purpose

Expresses SypHer in plant cells-biosensor for pH

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

SypHer

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDD363

Plasmid

#91829

Purpose

LoxP-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

LoxP-SEC-LoxP

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SHP2(T253M/Q257L)@pLKO-Trex-SBP

Plasmid

#85458

Purpose

SHP2 mutant gene expression

Depositor

Darrin Stuart

Publication

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

SBP Tag

Expression

Mammalian

Mutation

T253M/Q257L

Promoter

RSV

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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Tet-myr-CSK-GFP

Plasmid

#83470

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cells

Depositor

Barry Gumbiner

Publication

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

CSK (CSK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

doxycycline-inducible promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA

Plasmid

#81185

Purpose

Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c

Depositor

Jennifer Doudna

Publication

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Inserts

Cas5c
Cas8c
Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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acta2_GFP_pA_pDestTolpA2

Plasmid

#78684

Purpose

acta2:GFP transgenesis construct

Depositor

Sarah Childs

Publication

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Promoter

acta2

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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2PH-PLCd-APEX2-P2A-mKate2

Plasmid

#71284

Purpose

Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

2PH-PLCd-APEX2-P2A-mKate2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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Puro-iNgn3

Plasmid

#75337

Purpose

doxycycline inducible Ngn3 over-expression

Depositor

Danwei Huangfu

Publication

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

neurogenin 3 (Neurog3 Mouse)

Expression

Mammalian

Promoter

TRE-tight

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCZGY2139

Plasmid

#74111

Purpose

Membrane targeted PH-miniSOG entry clone for efficient ablation of cells when expressed under desired promoter.

Depositor

Andrew Chisholm

Publication

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

miniSOG

Use

Gateway topo cloning

Tags

PH

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS262

Plasmid

#73718

Purpose

C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Psnt-1::2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Promoter

Psnt-1

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mg319

Plasmid

#73367

Purpose

unc-70 tension sensor

Depositor

Miriam Goodman

Publication

Krieg et al Nat Cell Biol. 2014 Mar;16(3):224-33. do

Insert

unc-70::TSMod

Promoter

unc-70p

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAD/CMV/V5-DEST-Cdc42-2G

Plasmid

#68812

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

Depositor

Olivier Pertz

Publication

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Adenoviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GDI2

Plasmid

#67093

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GDI2 (GDI2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-SARA-GFP

Plasmid

#67410

Purpose

Mammalian expression of hSara with C-term GFP tag

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARA (SAR1B Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARL8B-Myc

Plasmid

#67362

Purpose

Mammalian expression of hArl8b with C-term Myc tag

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
ERalpha (Y537S mutant)-pcw107-V5

Plasmid

#64634

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

ESR1 (transcript variant 1) (ESR1 Human)

Use

Lentiviral

Tags

V5

Mutation

Y537S

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Hras (G12V)-pcw107

Plasmid

#64603

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

HRAS (HRAS Human)

Use

Lentiviral

Mutation

G12V

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMU-TLEr

Plasmid

#61187

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSLIK-Hygro-Myc-hAgo2

Plasmid

#60234

Purpose

Express HA-tagged wild-type Ago2 in mammalian cells in a Dox-inducible manner

Depositor

Zissimos Mourelatos

Publication

Liu et al Mol Cell. 2012 May 25;46(4):507-17. doi:

Insert

Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
LTN1_pCSdest

Plasmid

#53855

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

LTN1 (LTN1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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