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Human ICAM-2

Plasmid

#8634

Depositor

Timothy Springer

Article

Staunton et al Nature 1989 May 4;339(6219):61-4.

Insert

ICAM-2 (ICAM2 Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
SPLICS Po-Mt Long P2A

Plasmid

#164115

Purpose

Detect the long Peroxisomes-mitochondria contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Po-Mt Long P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-yellow pre-eGRASP(p30)

Plasmid

#111587

Purpose

An AAV vector that expresses yellow pre-eGRASP(p30) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p30)

Use

AAV

Promoter

CaMKIIa

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GATA1

Plasmid

#85693

Purpose

Mammalian expression of mouse GATA1

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-FKBP:mCherry-Rab5a

Plasmid

#72901

Purpose

Early endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab5a (RAB5A Human)

Use

Lentiviral

Tags

FKBP(F36V) and mCherry

Expression

Mammalian

Mutation

fragment a.a. 2-215

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-DHFR

Plasmid

#80329

Purpose

Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

DHFR-TSc3

Use

CRISPR

Promoter

DHFR-TS 5' UTR

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HshnRNPE1

Plasmid

#64921

Purpose

Expresses V5- and SBP-tagged hnRNPE1 in mammalian cells

Depositor

Elisa Izaurralde

Insert

hnRNPE1 (PCBP1 Human)

Tags

V5 and SBP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pBI-MCS-EYFP

Plasmid

#58855

Purpose

The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

enhanced yellow fluorescent protein

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pri-let-7a-1

Plasmid

#51377

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7a-1 (MIRLET7A1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-hGRK4

Plasmid

#32690

Depositor

Robert Lefkowitz

Article

Premont et al J Biol Chem. 1996 Mar 15;271(11):6403-10

Insert

GRK4 (GRK4 Human)

Expression

Mammalian

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only
RCAS(B)-Flag-PLZF

Plasmid

#34887

Depositor

Peter Vogt

Article

Shi et al Int J Cancer. 2009 Oct 1;125(7):1558-65.

Insert

PLZF (PLZF, ZBTB16 Human)

Use

Retroviral; Avian expression

Tags

FLAG

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only
myc-mULK1

Plasmid

#31960

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (Ulk1 Mouse)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pIC-Cre

Plasmid

#19131

Depositor

Klaus Rajewsky

Article

Gu et al Cell. 1993 Jun 18. 73(6):1155-64.

Insert

Cre recombinase (cre Bacteriophage P1)

Expression

Bacterial

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-5a

Plasmid

#17996

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5a (Wnt5a Mouse)

Use

Retroviral

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pGL3-NRASlong

Plasmid

#13797

Depositor

Frank Slack

Article

Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

Insert

NRAS (NRAS Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
pFlagCMV2-UbcM8

Plasmid

#12440

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

UbcM8 (Ube2l6 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-2T-Rac1-T17N

Plasmid

#12978

Depositor

Gary Bokoch

Insert

Rac1 DN (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

T17N Dominant negative

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
p3990 pET ubcH8

Plasmid

#8650

Depositor

Peter Howley

Article

Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

Insert

UbcH8 (UBE2L6 Human)

Tags

His

Expression

Bacterial

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
HA-BirA*-pDEST-N-pcDNA5/FRT/TO

Plasmid

#118375

Purpose

Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-BirA*

Expression

Mammalian

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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