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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 79,196 results for: rest

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Showing: 9841 - 9860 of 79196 results

  1. pEGFP-rNFM

    Plasmid
    #32907
    Purpose
    Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of rat neurofilament protein M
    Depositor
    Anthony Brown
    Publication
    Wang et al Nat Cell Biol. 2000 Mar;2(3):137-41.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-rat NFM fusion (Nefm Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    32907-plasmid-map-sequence-id-38889

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  9. GPR132-Tango

    Plasmid
    #66315
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR132 (GPR132 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  10. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Publication
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  12. pcDNA3-EKAR4

    Plasmid
    #174437
    Purpose
    Improved cyan/yellow FRET-based ERK kinase activity reporter.
    Depositor
    Jin Zhang
    Publication
    Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EKAR4
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174437-plasmid-map-sequence-id-492002

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  13. pcDNA3.1.Gαi3-LgB91

    Plasmid
    #134359
    Purpose
    Nanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gαi3-LgB91 (GNAI3 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134359-plasmid-map-sequence-id-306392

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  17. mCherry-2xCL-YFP-Vpr

    Plasmid
    #105215
    Purpose
    Bi-functional Fluorescent reporter protein to visualize HIV-1 fusion and core-trafficking
    Depositor
    Gregory Melikyan
    Publication
    Sood et al J Biol Chem. 2017 Oct 18. pii: M117.8180
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Vpr
    Tags
    PC2x (SQNY/IRKVL), eYFP, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105215-plasmid-map-sequence-id-203124

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  18. p7SL-neo-TET

    Plasmid
    #51285
    Purpose
    expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Publication
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    7SL RNA (RN7SL2 Human)
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Promoter
    7SL upstream pollII enhancer
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51285-plasmid-map-sequence-id-78367

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Showing: 9841 - 9860 of 79196 results