We narrowed to 9,922 results for: tre promoter
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Plasmid#227335PurposeDonor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)DepositorInsertH3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX458-Dual-Guide-Donor-ShuttleOnly
Plasmid#171098PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRMutationXbaI site added upstream of U6 promotor. GtoC mu…Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 BA.2 Spike
Plasmid#185604PurposeExpresses codon-optimized full length SARS-CoV-2 BA.2 SpikeDepositorInsertSARS-CoV-2 BA.2 Spike (S )
ExpressionMammalianMutationT19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…Promoterchicken β-actin promoter, CMV enhancerAvailable SinceJuly 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS SARS-CoV-2 BA.1 Spike
Plasmid#185452PurposeExpresses codon-optimized full length SARS-CoV-2 BA.1 SpikeDepositorInsertSARS-CoV-2 Spike BA.1 (S )
ExpressionMammalianMutationContains the following mutations: A67V, Δ69-70, T…Promoterchicken β-actin promoter, CMV enhancerAvailable SinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a_SYK
Plasmid#224240PurposeExpression of human SYK Kinase in E. coliDepositorAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PTK2b
Plasmid#224237PurposeExpression of human PTK2b Kinase in E. coliDepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET11a_PTK2b_Kinase
Plasmid#224242PurposeExpression of human PTK2b Kinase domain in E. coliDepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pScaffold-H1 donor
Plasmid#118152PurposePCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoterDepositorTypeEmpty backboneExpressionMammalianAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
ceOtub1
Plasmid#37196DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
252 pTol2-MTDest
Plasmid#73485Purposepromoterless Tol2 vector with 6xmyc tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signalDepositorInsert6xmyc/attR1/attR2
Available SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
366 pTol2-CherryDest
Plasmid#73487Purposepromoterless Tol2 vector with mCherry tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signalDepositorInsertmCherry-attR1/attR2
MutationH30R (please see depositor comments below)Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE8.20m-eVRQR-P2A-EGFP (LLH569)
Plasmid#242656PurposeCMV promoter expression plasmid for human codon optimized ABE8.20 A-to-G base editor with neVRQR(D10A) and P2A-EGFPDepositorInsertpCMV-ABE8.20m-VRQR-S55R-P2A-EGFP
UseCRISPRTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationABE8.20 mutations in TadA, eVRQR mutations in SpC…PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE8e-eVRQR-P2A-EGFP (HES1425)
Plasmid#242657PurposeCMV promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with neVRQR(D10A) and P2A-EGFPDepositorInsertpCMV-ABE8e-SpCas9-VRQR(S55R)-P2A-EGFP
UseCRISPRTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA, eVRQR mutations in SpCas…PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)
Plasmid#242660PurposeCBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassetteDepositorInsertpAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
UseAAV and CRISPRTagsBPNLSMutationVRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…PromoterCBhAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
N-term AAV-ABE8e-SpCas9(S55R) (CA21)
Plasmid#242659PurposeCBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructsDepositorInsertpAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSMutationSpCas9(S55R)PromoterCBhAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE8.8m-SpG-P2A-EGFP (BKS965)
Plasmid#242652PurposeCMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpG(D10A) and P2A-EGFPDepositorInsertpCMV-ABE8.8m-SpG-P2A-EGFP
UseCRISPRTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationABE8.8 mutations in TadA, SpG mutations in SpCas9…PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9-VRQR(+L1111R)-P2A-EGFP (LRF167)
Plasmid#242673PurposeCMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111R)-BPNLS(SV40)-3xFLAG-P2A-EGFPDepositorInsertpCMV-SpCas9-VRQR(L1111R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
UseCRISPRTagsBPNLSExpressionMammalianMutationVRQR + L1111R mutations in SpCas9(L1111R/D1135V/G…PromoterCMV and T7Available SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9-VRQR(+A1322R)-P2A-EGFP (LRF59)
Plasmid#242679PurposeCMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1322R)-BPNLS(SV40)-3xFLAG-P2A-EGFPDepositorInsertpCMV-SpCas9-VRQR(A1322R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
UseCRISPRTagsBPNLSExpressionMammalianMutationVRQR + A1322R mutations in SpCas9(D1135V/G1218R/A…PromoterCMV and T7Available SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
C-term AAV-eVRQR-ACTA2-gRNA-A4 (mouse) (LLH310)
Plasmid#242662PurposeCBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and mouse gRNA-A4DepositorInsertpAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_mouse_NGA_gRNA-pU6
UseAAV and CRISPRTagsBPNLSMutationVRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…PromoterCBhAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
C-term AAV-eVRQR-ACTA2-gRNA-A4 (human) (LLH301)
Plasmid#242661PurposeCBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and human gRNA-A4DepositorInsertpAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_human_NGA_gRNA-pU6
UseAAV and CRISPRTagsBPNLSMutationVRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…PromoterCBhAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only