We narrowed to 11,712 results for: NSI

1. pNBU2_erm-TetR-P1T_DP-GH023

   Plasmid

   #90324

   Purpose

   Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSR33.4r

   Plasmid

   #63198

   Purpose

   Expresses Cph8, Ho1, and PcyA constitutively

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   Cph8
   Ho1
   PcyA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR SFFVp Phy-mCherry-CAAX

   Plasmid

   #50839

   Purpose

   Optogenetic PI3K activation system; membrane component

   Depositor

   Wendell Lim

   Article

   Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

   Insert

   PhyB 1-908
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   mCherry

   Promoter

   SFFV

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAJE-Ma pylRS nitroY/haloY-F5

   Plasmid

   #225684

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

   Depositor

   Ryan Mehl

   Inserts

   M. alvus PylRS 3-NY/HaloY F5
   M. alvus Pyl-tRNA(6)
   

   Use

   Methanomethylophilus alvus

   Mutation

   CTG125CTT, N166A, V168S, A223C, W239R

   Promoter

   GlnS and Lpp

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-Sestrin2 (E451Q)

   Plasmid

   #237420

   Purpose

   Transient expression of N-terminally HA-tagged human Sestrin2 (E451Q)

   Depositor

   David Sabatini

   Article

   Valenstein et al Nature. 2025 Aug 20. doi: 10.1038/s41586

   Insert

   Sestrin2 (SESN2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E451Q

   Available Since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSR43.6r

   Plasmid

   #63197

   Purpose

   Expresses CcaS, Ho1, and PcyA constitutively

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   CcaS
   Ho1
   PcyA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dazl-CPG-TV

   Plasmid

   #70144

   Purpose

   a targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA

   Depositor

   Rudolf Jaenisch

   Article

   Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

   Insert

   5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL
   

   Use

   Mouse Targeting and Synthetic Biology

   Available Since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRE2_CMV_Mex3a_t2a_mCherry

   Plasmid

   #223244

   Purpose

   tetO responsive expression of Mex3a in mammalian cells with t2a cleaved mCherry

   Depositor

   Stavros Lomvardas

   Insert

   Mex3a (Mex3a Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   amino acids conserved, underlying DNA sequence re…

   Promoter

   CMV

   Available Since

   May 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3

   Plasmid

   #214926

   Purpose

   Expresses presynaptic non-responsive control

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (synapto).cpsfGFP.miRFP670nano3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CamKII

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCpfSA

    Plasmid

    #187890

    Purpose

    CRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus

    Depositor

    Quanjiang Ji

    Article

    Wang et al ACS Synth Biol. 2022 Sep 16;11(9):3049-3

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p-dCas9-JMJD2A-Hygro

    Plasmid

    #104410

    Purpose

    transient expression of dCas9-JMJD2A fusion protein

    Depositor

    Stephan Beck

    Insert

    JMJD2A (KDM4A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SOX2se-CPG-TV

    Plasmid

    #70155

    Purpose

    a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
    

    Use

    Mouse Targeting and Synthetic Biology

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-TRE-dTomato-GCaMP6s

    Plasmid

    #99864

    Purpose

    expresses dTomato and GCaMP6s under control of the Tet-off system

    Depositor

    Venkatesh Murthy

    Article

    Wallace et al Elife. 2020 Mar 9;9. pii: 50531. doi: 10

    Insert

    dTomato, GCaMP6s
    

    Promoter

    Tet-responsive promoter

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. HRE-doUnaG

    Plasmid

    #201713

    Purpose

    Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Expression

    Mammalian

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-TrxRFP1

    Plasmid

    #98996

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP
    

    Tags

    His-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRK669

    Plasmid

    #71313

    Purpose

    Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Expression

    Bacterial

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC1802_pmax-RBFOX1N-dCasRx-C

    Plasmid

    #118635

    Purpose

    Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    RBFOX1N-dCasRx-C
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXW117Ptet2-gfp

    Plasmid

    #160818

    Purpose

    aTc-responsive single-layered circuit

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

    Insert

    PJ23117-30tetR-t-Ptet2-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PJ23117, Ptet2

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEVOL-pylT-AznLRS

    Plasmid

    #172482

    Purpose

    For E.Coli expression, 306L309L348I384F411W

    Depositor

    Wenshe Liu

    Article

    Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

    Insert

    AznLRS and pylTcua
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. U6.3>gRNA.f+e

    Plasmid

    #99139

    Purpose

    Empty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoter

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    gRNA
    

    Use

    CRISPR

    Promoter

    chicken U6.3

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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