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pGDP3 apmA

Plasmid

#112881

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

apmA (apmA Staphylococcus aureus)

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm6b(1025-End)-pA

Plasmid

#100278

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Tags

Flag epitope tag

Expression

Mammalian

Mutation

catalytic domain (1025aa-End)

Promoter

T7

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pPSup_35Senh(fwd)_35Spr+synJ

Plasmid

#149422

Purpose

Plasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Insert

35S core enhancer

Expression

Plant

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin4A

Plasmid

#41914

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL4A (CUL4A Human)

Use

Lentiviral

Available Since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin2

Plasmid

#41912

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL2 (CUL2 Human)

Use

Lentiviral

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RNMT

Plasmid

#112705

Purpose

Expresses N-terminally FLAG-tagged human RNMT in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Promoter

myo-3 and unc-119

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pEY121

Plasmid

#223468

Purpose

sra-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

sra-6 (sra-6 Nematode)

Expression

Worm

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-NG-PE2-BSD

Plasmid

#176933

Purpose

Expresses PE2-NG variant in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Insert

PE2-NG variant

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-His-hCE

Plasmid

#112710

Purpose

Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNGTT (RNGTT Human)

Tags

6xHis and Stop codons placed after RNGTT sequence…

Expression

Bacterial

Mutation

R594H

Promoter

T7

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin4B

Plasmid

#41915

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL4B (CUL4B Human)

Use

Lentiviral

Available Since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR MCS R4-R3

Plasmid

#62086

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV WT SPOP 28-359

Plasmid

#194115

Purpose

Wild type His-SUMO SPOP expression plasmid

Depositor

Tanja Mittag

Article

Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

Insert

SPOP (SPOP Human)

Tags

6His-SUMO-TEV

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLPP6

Plasmid

#209968

Purpose

Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter Dropout (sfGFP)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

Plasmid

#199351

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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3xFLAG-SENP6

Plasmid

#42887

Depositor

Edward Yeh

Article

Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

Insert

SENP6 (SENP6 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Please see depositor comments

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (S939A)

Plasmid

#14132

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A.

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
anti-Notch2_B6-pBIOCAM5

Plasmid

#39347

Depositor

John McCafferty

Article

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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AsdCas12a VPR

Plasmid

#188512

Purpose

Expresses FLAG tagged AsdCas12a VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D908A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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p4305

Plasmid

#198150

Purpose

bacterial expression of sifA::HaloTag fusion

Depositor

Michael Hensel

Article

Goser et al Nat Commun. 2023 Mar 4;14(1):1240. doi:

Insert

PsifA sifA::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)

Tags

HaloTag-HA

Expression

Bacterial

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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