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pGSTag BimL

Plasmid

#24251

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (BCL2L11 Human)

Tags

GST

Expression

Bacterial

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pCaUra3-CUbo-AID*-GFP

Plasmid

#212775

Purpose

C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRTP801/TA-ODD

Plasmid

#206212

Purpose

An expression vector of hypoxia-responsive transactivator under control of RTP801 promoter

Depositor

Masamichi Kamihira

Article

Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

Insert

tetracycline-responsive transactivator fused with oxygen-dependent degradation domain

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCIS-krPAM2

Plasmid

#25457

Depositor

Richard Mains

Article

Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

Insert

PAM2 (Pam Rat)

Expression

Mammalian

Mutation

Exon A deleted (exon A extends from nt 1475 to n…

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pASKt15C+SrtA5

Plasmid

#64985

Purpose

Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

Plasmid

#21800

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD14 (Psmd14 Mouse)

Tags

GST and TEV

Expression

Mammalian

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pJAG98-mTurquoise2-Nuf2

Plasmid

#80760

Purpose

Express Nuf2 N-terminally labeled with mTurquoise2

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

Nuf2 (NUF2 Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pME-puro-CLIP-FLAG-CD59

Plasmid

#50375

Purpose

Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

CLIP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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GST-U1C

Plasmid

#1840

Depositor

Philip Leder

Article

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

U1C (SNRPC Human)

Tags

GST

Expression

Bacterial

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pCMB-MTUBr

Plasmid

#61197

Purpose

Fluorescent reporter for microtubules mCherry-MAP4-MBD expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MAP-MBD

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

Plasmid

#31478

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 K227M T2A BFP

Use

Lentiviral

Tags

HA and NLS

Mutation

F13Y, S111Y, K227M = Y2 nickase "reo" …

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pSV(X) C/EBPbeta

Plasmid

#13393

Depositor

Richard Schwartz

Article

Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

Insert

C/EBPbeta (Cebpb Rat)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
Phat2-hs-cPla2-C2-EGFP-GH

Plasmid

#187088

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000116711 (PLA2G4A Human)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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SGL40C-H1.EFS.RFP657

Plasmid

#69148

Purpose

Advanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

sgRNA
EFS
tagRFP657

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCB150-2_GFP-EB1

Plasmid

#46364

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

EB1 (MAPRE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-2T SHP1 CS

Plasmid

#8595

Depositor

Ben Neel

Article

Timms et al Mol Cell Biol 1998 Jul;18(7):3838-50.

Insert

SHP1 (PTPN6 Human)

Tags

GST

Expression

Bacterial

Mutation

C453S (catalytically inactive)

Available Since

June 21, 2005

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-EPSIN 1

Plasmid

#22225

Depositor

Pietro De Camilli

Article

Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

Insert

Epsin 1 (Epn1 Rat)

Tags

Xpress

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
TN-XXL pRSETB

Plasmid

#45796

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Insert

TN-XXL

Tags

6xHIS, ECFP, and YFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
pSV(X) C/EBPalpha

Plasmid

#13392

Depositor

Richard Schwartz

Article

Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

Insert

C/EBPalpha (Cebpa Rat)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
Dlx6

Plasmid

#15539

Depositor

John Rubenstein

Article

Liu et al Dev Dyn. 1997 Dec . 210(4):498-512.

Insert

Dlx6 (Dlx6 Mouse)

Expression

Bacterial

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only