We narrowed to 11,712 results for: NSI

1. pBK-Ma pylRS nitroY/haloY-F5

   Plasmid

   #212125

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.

   Depositor

   Ryan Mehl

   Insert

   M. alvus PylRS 3-NY/HaloY F5
   

   Expression

   Bacterial

   Mutation

   CTG125CTT, N166A V168S, A223C, W239R

   Promoter

   GlnS (constitutive)

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMUT2-pFF-41.5-mCherry

   Plasmid

   #192863

   Purpose

   pMUT2 based vector for sensing oxygen in E. coli Nissle 1917

   Depositor

   Morten Sommer

   Article

   Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

   Insert

   Oxygen inducible promoter
   

   Expression

   Bacterial

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFBP_promoter_acceptor

   Plasmid

   #189920

   Purpose

   Binary vector to accept a custom promoter sequence to drive fungal luciferase expression by Agrobacterium-mediated transient transformation

   Depositor

   Adam Steinbrenner

   Article

   Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35S

   Plasmid

   #211837

   Purpose

   Plant transient expression cassette for HR1b-GFP fusion under UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Plant

   Promoter

   UBIQUITIN10

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHis17_ParM _1

   Plasmid

   #78201

   Purpose

   Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

   Depositor

   Martin Webb

   Article

   Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

   Insert

   parM (K33A I27C T174A T175N C287A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   K33A I27C T174A T175N C287A

   Promoter

   unknown

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19/mChe-C

   Plasmid

   #183160

   Purpose

   Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-cytoFLARE1.0/1.1-TF

   Plasmid

   #234516

   Purpose

   Transcriptional reporter for detecting calcium transients in neuronal cultures (transcription factor)

   Depositor

   Wenjing Wang

   Article

   Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

   Insert

   ERT2-MK2-hLOV-TEVcs-FLAG-tTA
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pYL322d1/N-eGFP

   Plasmid

   #183151

   Purpose

   Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19/eCFP-C

   Plasmid

   #183156

   Purpose

   Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFP

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJKR-H-cdaR

    Plasmid

    #62557

    Purpose

    Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Inserts

    beta lactamase
    sfGFP
    CdaR
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPRIME-TET-GFP-recipient

    Plasmid

    #11655

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-b3STOPb3-ZsGreen

    Plasmid

    #51266

    Purpose

    B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    b3STOPb3-ZsGreen
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFH2.4_mCherry-MMLV_Psi

    Plasmid

    #205522

    Purpose

    Express mCherry-Psi (export tag consisting of MMLV Psi)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pESC-URA-GFP-Ubc9ts

    Plasmid

    #20369

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Y68L This mutation renders Ubc9 temperature-sens…

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pRGD-TcR

    Plasmid

    #74110

    Purpose

    Plasmid containing tetracycline resistance gene cassette flanked by multiple restriction sites

    Depositor

    Hans-Martin Fischer

    Article

    Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

    Insert

    tetA(C)
    

    Use

    Bacterial vector based on pmb1 replicon

    Mutation

    C-to-T transition at pos. no. 1049 of tetA(C) lea…

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. LRP6-pCS2-VSVG

    Plasmid

    #27282

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Available Since

    March 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. 625 pTol2-tp1bglob:egfp

    Plasmid

    #73586

    Purpose

    Tol2 vector with Notch responsive tp1 element driving expression of EGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Development. 2014 Apr;141(7):1544-52. do

    Insert

    tp1 element driving EGFP
    

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCBFRE-(mt)-luc

    Plasmid

    #26896

    Depositor

    Nicholas Gaiano

    Article

    Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

    Insert

    CBFRE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutated CBF1 sites (see note)

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pBTSG

    Plasmid

    #159420

    Purpose

    Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Tags

    polyhistidine (poly-His)

    Expression

    Mammalian

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTR47m4

    Plasmid

    #104068

    Purpose

    Luciferase Reporter for Formaldehyde Sensing

    Depositor

    David Liu

    Article

    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

    Inserts

    xluxAB
    frmR
    frm Promoter m4
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Bacterial

    Mutation

    23r and 25th nucleotides in sequence mutated from…

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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