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Lenti.pFOXA2.5'.GFP

Plasmid

#59407

Purpose

expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

Depositor

Kai-Christian Sonntag

Article

Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

Insert

approx. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

FOXA2

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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FAS_pLX307

Plasmid

#98334

Purpose

Lentiviral expression of FAS

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

FAS (FAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3 3'UTR reporter MUT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107010

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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DDX21

Plasmid

#155510

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX21 (DDX21 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

T232A

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS-FLAG-cGAS

Plasmid

#127659

Purpose

Over-express EGFP-NLS-FLAG-cGAS

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

EGFP-NLS-Flag

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-HTR6-SNAPf-TRIP

Plasmid

#189617

Purpose

Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6-TRIP_alpha

Use

AAV

Tags

alphaTag in the N-term of HTR6, SNAP-tag in the C…

Promoter

TRE

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO (pEHA1339)

Plasmid

#209088

Purpose

Backbone plasmid for Tet Responsive transgene expression, FRT integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

Plasmid

#185916

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8.20m-nSpG-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

Plasmid

#224123

Purpose

Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-mutant (Q20A_Y23A_Y24F_F35A)

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Q20A_Y23A_Y24F_F35A in La(SSB)1-194

Promoter

CMV

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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BRAF_pLX307

Plasmid

#98322

Purpose

Lentiviral expression of BRAF

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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shBIRC5 # 2

Plasmid

#42554

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shBIRC5 (BIRC5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
shBIRC5 # 1

Plasmid

#42553

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shBIRC5 (BIRC5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
Lenti-sgTnr#2/Cre

Plasmid

#193245

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tnr gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgTnr#2 (Tnr Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 AIF 1-90 mCherry FRB

Plasmid

#67530

Purpose

For expression of mCherry fused FRB to the mitochondrial innermembrane

Depositor

Stephen Tait

Article

Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

Inserts

AIF a.a. 1 - 90
mCherry
FRB

Tags

AIF 1 - 90, FRB, and mCherry

Expression

Mammalian

Mutation

T->L

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCX-HO1-2A-EGFP

Plasmid

#74672

Purpose

Mammalian expression of HO1 and EGFP

Depositor

Roberto Giovannoni

Article

Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

Insert

HO1 (HMOX1 Human)

Tags

2A-EGFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1B-WT-Flag-UTR

Plasmid

#183239

Purpose

Expression of FLAG tagged HNF1B

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox B (HNF1B Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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BCL2L1_pLX307

Plasmid

#98323

Purpose

Lentiviral expression of BCL2L1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5 + PGK-blast

Plasmid

#167818

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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INTS6

Plasmid

#155681

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

INTS6 (INTS6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-KIF2C

Plasmid

#205996

Purpose

inducible expression of HA-KIF2C

Depositor

Floris Foijer

Article

Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

Insert

KIF2c (KIF2C Human)

Tags

HA

Expression

Mammalian

Promoter

pTight

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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