Addgene

Showing: 81 - 100 of 1631 results

Results per page:

pOG2002

Plasmid

#49514

Purpose

TALEN backbone for expression of TALE(deltaRDV)-FokI AS in C. elegans

Depositor

Guangshuo Ou

Article

Cheng et al Nat Biotechnol. 2013 Oct;31(10):934-7. d

Type

Empty backbone

Use

Tags

3xFLAG, FokI-AS, and SV40 NLS

Expression

Worm

Mutation

Promoter

hsp-16.2

Availability

Academic Institutions and Nonprofits only

Enlarge
pOG2003

Plasmid

#49516

Purpose

TALEN backbone for expression of TALE(deltaRDV)-FokI RR in C. elegans

Depositor

Guangshuo Ou

Article

Cheng et al Nat Biotechnol. 2013 Oct;31(10):934-7. d

Type

Empty backbone

Use

Tags

FokI-RR, HA, and SV40NLS

Expression

Worm

Mutation

Promoter

hsp-16.2

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLGCV

Plasmid

#49862

Purpose

Expression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).

Depositor

Anne Glover, Jonathan Pettitt

Article

Lagido et al BMC Physiol. 2008 Apr 2;8:7. doi: 10.118

Insert

firefly luciferase luc+ fused to GFP (S65C)

Use

Tags

GFP (S65C)

Expression

Worm

Mutation

Promoter

sur-5

Availability

Academic Institutions and Nonprofits only

Enlarge
pHKMC1: Empty sgRNA for Cloning

Plasmid

#67720

Purpose

Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

Depositor

Monica Colaiacovo

Article

Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

PU6

Availability

Academic Institutions and Nonprofits only

Enlarge
pEft-3::oxCerulean-oxVenus::Lgg-1

Plasmid

#68040

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed ubiquitously, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::oxCerulean-oxVenus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pVha-6::oxCerulean-oxVenus::Lgg-1

Plasmid

#68041

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in intestines, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pVha-6::oxCerulean-oxVenus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDpy-7::oxCerulean-oxVenus::Lgg-1

Plasmid

#68042

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in hypodermis, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pDpy-7::oxCerulean-oxVenus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pMyo-2::oxCerulean-oxVenus::Lgg-1

Plasmid

#68043

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the pharynx, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pMyo-2::oxCerulean-oxVenus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pMyo-3::oxCerulean-oxVenus::Lgg-1

Plasmid

#68044

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in body wall muscle, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pMyo-3::oxCerulean-oxVenus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pRab-3::Cerulean-Venus::Lgg-1

Plasmid

#68045

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the neurons, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pRab-3::Cerulean-Venus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Gatewayp221oxCerulean-oxVenus

Plasmid

#68047

Purpose

oxCerulean-oxVenus in Gateway [1-2] plasmid p221

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

p221oxCerulean-oxVenus

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEft-3::Cerulean-Venus

Plasmid

#68048

Purpose

Ubiquitious expression of oxCerulean-oxVenus (dFP)

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Use

Tags

Expression

Worm

Mutation

Promoter

rab-3

Availability

Academic Institutions and Nonprofits only

Enlarge
Prab-3::NLS::GFP

Plasmid

#68120

Purpose

Nuclear Localized GFP for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

GFP

Use

Tags

Expression

Worm

Mutation

Promoter

rab-3

Availability

Academic Institutions and Nonprofits only

Enlarge
pJA327

Plasmid

#74486

Purpose

superfolder GFP (myo-3::sfGFP::let-858, using backbone as in L3785)

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

superfolder GFP

Use

Tags

Expression

Worm

Mutation

Promoter

myo-3

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM32DEST

Plasmid

#74747

Purpose

Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

Depositor

Keith Yamamoto, Jordan Ward

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

Gateway cassette
QF-GR (NR3C1 Synthetic, Human, Neurospora crassa)

Use

Tags

Fusion of the Neurospora crassa QF activator to t…

Expression

Worm

Mutation

Promoter

No promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM34DEST

Plasmid

#74756

Purpose

Encodes for the 5X repeat of the QUAS response element, followed by the delta pes-10 minimal promoter

Depositor

Keith Yamamoto, Jordan Ward

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Insert

Gateway cassette

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM48DEST

Plasmid

#74757

Purpose

Encodes for the QS repressor gene, splice linker, and mCherry reporter gene

Depositor

Keith Yamamoto, Jordan Ward

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

QS
Gateway cassette

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM59

Plasmid

#74785

Purpose

Expresses 3XFLAG::GR LBD::DAF-16A from the hsp16.48 promoter

Depositor

Keith Yamamoto, Jordan Ward

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Insert

3X FLAG::GR LBD::DAF-16A (daf-16, NR3C1 Synthetic, Human, Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

hsp16.48

Availability

Academic Institutions and Nonprofits only

Enlarge