We narrowed to 505 results for: SPY

1. SpyCatcher003 S49C

   Plasmid

   #133448

   Purpose

   Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Contains S49C mutation

   Promoter

   T7

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyTag003-MBP

   Plasmid

   #133450

   Purpose

   Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to N-terminus of MBP via a GSGE…

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST14-SpyCatcher002

   Plasmid

   #102827

   Purpose

   Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-SpyTagMBP

   Plasmid

   #35050

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyTagMBP
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Simple peptide tag added to the N-terminus of MBP…

   Promoter

   T7 promoter

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST14-SpyCatcher

   Plasmid

   #35044

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyCatcher
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Deletion of C-terminal till I113, and point mutat…

   Promoter

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. LLP786_SpyCatcher-EZH2-FL-CO

   Plasmid

   #211779

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-KRAB
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect KRAB e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEC2483 (pSpy1C)

   Plasmid

   #220248

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_NESPylRS(AF)_hU6tRNAPyl

   Plasmid

   #223508

   Purpose

   PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

   Inserts

   NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y306A; Y384F

   Promoter

   CVM, SP6 and U6

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpyTag-sfGFP

   Plasmid

   #184226

   Purpose

   Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpyTag-sfGFP
   

   Use

   Tags

   His6, SpyTag, and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP776_SpyCatcher-p65HSF1-CO

    Plasmid

    #211769

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SpyCatcher-p65HSF1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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