3,636 results

1. W45A mutant of H3K9me3 biosensor

   Plasmid

   #120808

   Purpose

   FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

   Promoter

   CMV

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. 20XUAS IVS ChrimsonR mCherry_tr

   Plasmid

   #111546

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc V5 dDispatched WT HA

   Plasmid

   #126405

   Purpose

   Expression of V5 dDispatched WT HA in Drosophila cells lines.

   Depositor

   Stacey Ogden

   Article

   Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

   Insert

   d-Dispatched1 (disp Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. dVGlut-Gal80

   Plasmid

   #52555

   Purpose

   Expresses Gal80 under vesicular glutamate transporter promoter

   Depositor

   Leslie Vosshall

   Insert

   VGlut-Gal80 (VGlut, GAL80 Fly, Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila Synthetic Core Promoter

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJC-repo1.9kb-TALE4-VP64-P10

   Plasmid

   #104612

   Purpose

   Expresses TALE4 under the control of a 1.9kb repo enhancer element

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   1.9kb repo enhancer element (repo Fly)
   

   Use

   TALEN

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFD2-dU6:2gRNA

   Plasmid

   #49409

   Purpose

   expression of gRNA under control of the Drosophila U6:2 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-2:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-2

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

   Plasmid

   #217512

   Purpose

   KD Recombinase dependent conditional smGdP-10XOllas cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
   

   Use

   Tags

   smGdP-10XOllas

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PUC19_C_tag

   Plasmid

   #186659

   Purpose

   Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   C-terminal 3xFlag-3xHA Tag
   

   Use

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

   Plasmid

   #63168

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-V5"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   GAL4-responsive UAS hsp70

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. w+attB

    Plasmid

    #30326

    Depositor

    Jeff Sekelsky

    Insert

    phiC31 attB, hsp70::w
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS-KS-attB1-2-PT-SA-SD-1-GFP11

    Plasmid

    #172068

    Purpose

    Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Renilla luciferase-Pol III

    Plasmid

    #37380

    Depositor

    Norbert Perrimon

    Article

    Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

    Insert

    PolIII-Renilla control reporter (RpIII128 Fly)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA PolIII 128 subunit (RpIII128)

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCB19

    Plasmid

    #194757

    Purpose

    EGFP-tagged GluRIIA-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Tags

    EGFP-Tag

    Expression

    Mutation

    none

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCB20

    Plasmid

    #194758

    Purpose

    ALFA-tagged GluRIIA-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Tags

    ALFA-Tag

    Expression

    Mutation

    none

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LifeAct-MTED

    Plasmid

    #217498

    Purpose

    Expresses LifeAct-mScarlet-MTED for targeting fluorescent, MT depolymerizing peptide MTED to f-actin

    Depositor

    Erik Dent

    Article

    Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

    Insert

    LifeAct-mScarlet-MTED
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAC5.1-dCry-V5His

    Plasmid

    #136237

    Purpose

    Protein expression of D. melanogaster (Dm) cryptochrome with C-terminal V5 and His tags

    Depositor

    Aziz Sancar, Steven Reppert

    Article

    Zhu et al Curr Biol. 2005 Dec 6;15(23):R953-4. doi

    Insert

    DmCry
    

    Use

    Tags

    His and V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid

    #126479

    Purpose

    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Inserts

    Or56a (Or56a Fly)
    Orco (Orco Fly)
    

    Use

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens and codon optim…

    Promoter

    CMV

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1A-Dm-miR-92a

    Plasmid

    #22767

    Depositor

    Elisa Izaurralde

    Article

    Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

    Insert

    micro RNA 92a (mir-92a Fly, primary transcript)
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

    Plasmid

    #63167

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-cMyc"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUASTLOTattB_VHH-GFP4::CD8::mCherry

    Plasmid

    #163917

    Purpose

    Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers

    Depositor

    Markus Affolter

    Article

    Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

    Insert

    Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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