3,636 results

1. pUAST-attB-yki:V5-S168A

   Plasmid

   #85617

   Purpose

   Expresses V5 tagged mutant yki in Drosophila.

   Depositor

   Kenneth Irvine

   Article

   Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

   Insert

   yki-V5-S168A
   

   Tags

   V5

   Expression

   Insect

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-Hrp48-ADARcd-V5

   Plasmid

   #81172

   Purpose

   identification of cell-specific targets of RNA binding proteins

   Depositor

   Michael Rosbash

   Article

   McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

   Insert

   Hrp48-ADARcd fusion (Hrb27C Fly)
   

   Expression

   Insect

   Promoter

   Drosophila metallothionein promoter

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3_NSnoFbox-vhhGFP4

   Plasmid

   #35580

   Depositor

   Markus Affolter

   Article

   Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

   Insert

   NSnoFbox-vhhGFP4 (slmb Fly)
   

   Expression

   Mammalian

   Mutation

   F box domain of slmb deleted.

   Available Since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-HemmarR

   Plasmid

   #31222

   Depositor

   Yuh-Nung Jan

   Article

   Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

   Insert

   CD4-tdTom (CD4 Fly, Human, Aequorea victoria)
   

   Tags

   CD4 and tdTomato

   Expression

   Insect

   Mutation

   Fusion of signal peptide of Drosophila Akh gene, …

   Available Since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHD-T2A-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199204

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int
   

   Expression

   Insect

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. ninaE-norpA

   Plasmid

   #26854

   Depositor

   Craig Montell

   Article

   Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

   Insert

   no receptor potential A (norpA Fly)
   

   Expression

   Insect

   Mutation

   V963A

   Available Since

   April 5, 2011

   Availability

   Academic Institutions and Nonprofits only

7. GFP-Rab32

   Plasmid

   #31744

   Depositor

   Ron Vale

   Article

   Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

   Insert

   Rab32 (Rab32 Fly)
   

   Tags

   GFP

   Expression

   Insect

   Promoter

   Actin

   Available Since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

8. Renilla luciferase-Pol III

   Plasmid

   #37380

   Depositor

   Norbert Perrimon

   Article

   Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

   Insert

   PolIII-Renilla control reporter (RpIII128 Fly)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   RNA PolIII 128 subunit (RpIII128)

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pUAS-syb:spGFP1–10

   Plasmid

   #73698

   Purpose

   Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP

   Depositor

   Marco Gallio

   Article

   Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

   Insert

   Neuronal-synaptobrevin:spGFP1–10 chimera
   

   Use

   Drosophila

   Expression

   Insect

   Available Since

   March 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFD2-dU6:2gRNA

    Plasmid

    #49409

    Purpose

    expression of gRNA under control of the Drosophila U6:2 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-2:gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6-2

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. w+attB

    Plasmid

    #30326

    Depositor

    Jeff Sekelsky

    Insert

    phiC31 attB, hsp70::w
    

    Use

    Drosophila transgenesis

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. SGyA

    Plasmid

    #160292

    Purpose

    Drives expression of Cas9 and a TdTomato fluorescent marker

    Depositor

    Omar Akbari

    Article

    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

    Inserts

    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    

    Tags

    T2A GFP

    Expression

    Insect

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 57F03_LexA-GAD

    Plasmid

    #83256

    Purpose

    Expresses LexA-GAD under the control of 57F03 enhancer

    Depositor

    Leslie Vosshall

    Article

    Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

    Insert

    57F03 enhancer
    

    Expression

    Insect

    Available Since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid

    #126479

    Purpose

    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Inserts

    Or56a (Or56a Fly)
    Orco (Orco Fly)
    

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens and codon optim…

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKC52

    Plasmid

    #226780

    Purpose

    Expresses Drosophila UNC-104(R348G) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (R348G) (unc-104 Fly)
    

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    changed Arginine 348 to Glycine

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKC50

    Plasmid

    #226778

    Purpose

    Expresses Drosophila UNC-104(V6M) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (V6M) (unc-104 Fly)
    

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    changed Valine 6 to Methionine

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKC28

    Plasmid

    #226776

    Purpose

    Expresses Drosophila UNC-104(G618R) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (G618R) (unc-104 Fly)
    

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    G618R

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

    Plasmid

    #63168

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-V5"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Promoter

    GAL4-responsive UAS hsp70

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTub-Wg

    Plasmid

    #64321

    Purpose

    Expresses Drosophila Wingless protein under the tubulin promotor

    Depositor

    Roel Nusse

    Article

    Ching et al J Biol Chem. 2008 Jun 20;283(25):17092-8

    Insert

    Wingless (wg Fly)
    

    Use

    Drosophila expression

    Promoter

    alpha-1 tubulin promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

20. W45A mutant of H3K9me3 biosensor

    Plasmid

    #120808

    Purpose

    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    

    Expression

    Mammalian

    Mutation

    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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