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Showing: 201 - 250 of 638 results

  13. p6XHis-T7(P266L)

    Plasmid
    #174866
    Purpose
    Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.
    Depositor
    Anna Pyle
    Article
    Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.
    Insert
    T7 RNA polymerase
    Use
    Tags
    6XHis-tag
    Expression
    Bacterial
    Mutation
    P266L
    Promoter
    lac promoter
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174866-plasmid-map-sequence-id-419578

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  18. pBRα-β flap (831-1057)

    Plasmid
    #53734
    Purpose
    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    alpha NTD-Beta flap fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    53734-plasmid-map-sequence-id-83920

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  25. pEV_S

    Plasmid
    #100266
    Purpose
    pDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coli
    Depositor
    Timothy Lu
    Article
    Cleto et al MBio. 2017 Oct 10;8(5). pii: e01474-17.
    Inserts
    entD (entD Escherichia coli)
    entC
    entE
    entB
    entA
    vibH
    vibF
    cos
    Use
    Synthetic Biology; Expression, pbr322
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100266-plasmid-map-sequence-id-193235

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  26. dSp-VP64-RTA

    Plasmid
    #99675
    Purpose
    Expresses dSp Cas9 fused at C term. To VP64 and RTA activation domains
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    Tags
    VP64-RTA
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    99675-plasmid-map-sequence-id-192088

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  40. dSp-Rta 125-190

    Plasmid
    #99664
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    Tags
    truncated RTA activation domain ( 125-190)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    99664-plasmid-map-sequence-id-192028

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  45. dSp-Rta 125-175

    Plasmid
    #99668
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    Tags
    truncated RTA activation domain (125-175)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99668-plasmid-map-sequence-id-189832

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  47. dSp-p65 (1-100)

    Plasmid
    #99660
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    Tags
    truncated p65 activation domain (1-100)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99660-plasmid-map-sequence-id-189821

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  48. dSp-p65 (50-150)

    Plasmid
    #99661
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    Tags
    truncated p65 activation domain (50-150)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99661-plasmid-map-sequence-id-189830

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  49. dSp-Rta 75-190

    Plasmid
    #99663
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    Tags
    truncated RTA activation domain (75-190)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99663-plasmid-map-sequence-id-192026

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  50. dSp-Rta 75-175

    Plasmid
    #99666
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    Tags
    truncated RTA activation domain (75-175)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99666-plasmid-map-sequence-id-192042

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Showing: 201 - 250 of 638 results