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Plasmid#220045PurposeMammalian expression of type III-A crRNA and Cas6 crRNA-processing ribonuclease from Streptococcus thermophilusDepositorInsertcrRNA; Cas6
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN052
Plasmid#220046PurposeMammalian expression of type III-A Csm complex from Streptococcus thermophilus without NLS tagsDepositorInsertFLAG-Csm1; FLAG-Csm2; FLAG-Csm3; FLAG-Csm4; FLAG-Csm5; FLAG-Cas6; crRNA; GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN099
Plasmid#220044PurposeMammalian expression of type III-A crRNA from Streptococcus thermophilusDepositorInsertcrRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pANG03
Plasmid#178085PurposeTetracycline resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG02.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterNAAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pANG01
Plasmid#178083PurposeCarbenicillin resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG02.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterNAAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pANG02
Plasmid#178084PurposeGentamycin resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG01 or pANG03.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterNAAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
PANK2_Halo_C_allele
Plasmid#178145PurposeDonor vector for endogenous tagging of human PANK2 at the C-terminus with halotagInsertHalotag (PANK2 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pANY8
Plasmid#164900PurposeContaining His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.DepositorInsertccdB cassette
UseTagsHis-ELP-Intein-tagExpressionBacterialMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pANY4
Plasmid#164897PurposeTwo valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.DepositorInsertccdB cassette
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pANTO3
Plasmid#131107PurposeTo generate a replicative plasmid carrying mycobacteriophage L5 integraseDepositorInsertmycobacteriophage L5 integrase
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SPANXN4_WT
Plasmid#81998PurposeGateway Donor vector containing SPANXN4, part of the Target Accelerator Plasmid Collection.DepositorInsertSPANXN4 (SPANXN4 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SPANXN4_p.P4Q
Plasmid#81262PurposeGateway Donor vector containing SPANXN4 , part of the Target Accelerator Plasmid Collection.DepositorInsertSPANXN4 (SPANXN4 Human)
UseGateway entry vectorTagsExpressionMutationP4QPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAN1578
Plasmid#127200PurposeProgesterone inducible expression of KeyA-BFPDepositorInsertpPAB1-Z3PM-tTDH1-pZ3-key_A_e18-mTagBFP2-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAN1646
Plasmid#127205PurposepTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLSDepositorInsertp7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAN1777
Plasmid#127206PurposeDual inducible expression of SynTF-mScarlet-nesSwitchA and KeyA-BFP-NLSDepositorInsertpGal1-NLS(activity4)-SynTF-mScarlet-1_nesSwitch_A_t9-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-N1-Tspan4
Plasmid#210323PurposeExpresses Tetraspanin-4 (TSPAN4) as migrasome markerDepositorInsertTetraspanin 4 (Tspan4 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Nuclear PancACe
Plasmid#215706PurposeExpresses acetyl-CoA biosensor in the nucleusDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and NLSExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mitochondrial PancACe
Plasmid#215710PurposeExpresses acetyl-CoA biosensor in the mitochondriaDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and mitochondrial localization sequence…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cytoplasmic PancACe
Plasmid#215708PurposeExpresses acetyl-CoA biosensor in the cytoplasmDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and cytoplasmic localization sequenceExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only