Showing: 81 - 92 of 92 results
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Plasmid#158478PurposeExpress nuclease dead Cas9 fused to KillerRed chromophore in mammalian cellsDepositorInsertdCas9-KR
UseCRISPRTagsKillerRedExpressionMammalianMutationCas9m4 (D10A+D839A+H840A+N863A)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC+] (AVA2626)
Plasmid#85444PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC+] (AVA2600)
Plasmid#85446PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCK301
Plasmid#87767PurposeE. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacIDepositorInsertPrhaBAD-sfGFP
UseSynthetic BiologyTags6xHis TagExpressionBacterialMutationPromoterPrhaBAD rhamnose-inducible promoter from E. coliAvailabilityAcademic Institutions and Nonprofits only -
JpExpress404 PTEN-LONG
Plasmid#49417PurposeBacterial Expression VectorDepositorInsertPTEN-Long (PTEN )
UseTagsExpressionBacterialMutationPromoterT5AvailabilityAcademic Institutions and Nonprofits only -
JpExpress404 PTEN
Plasmid#49420PurposeBacterial Expression VectorDepositorInsertPTEN (PTEN )
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
KRAS-V14I
Plasmid#154427PurposeV14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR
Plasmid#154826PurposeLanding pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)DepositorInsertsmCherry
NeoR
ZsGreen1-DR
UseCRISPR and Cre/LoxTagsPESTExpressionMammalianMutationPromoterCMV, EF1a, and SV40 early promoterAvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR
Plasmid#154827PurposeLanding pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)DepositorInsertsTagBFP
HygR
ZsGreen1-DR
UseCRISPR and Cre/LoxTagsPESTExpressionMammalianMutationPromoterCMV, EF1a, and SV40 early promoterAvailabilityAcademic Institutions and Nonprofits only -
KRAS-P34R
Plasmid#158527PurposeExpresses human KRAS P34R in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterT5AvailabilityAcademic Institutions and Nonprofits only -
pCK302
Plasmid#87768PurposeAs pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be usedDepositorInsertsPrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli
UseSynthetic BiologyTags6xHis TagExpressionBacterialMutationPromoterPrhaBAD rhamnose-inducible promoter from E. coli …AvailabilityAcademic Institutions and Nonprofits only -
KRAS-A146T
Plasmid#154426PurposeA146T Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterT5AvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 92 of 92 results