Addgene

Showing: 81 - 100 of 3852 results

Results per page:

Sort results by:

pES001 (pQacA-deGFP)

Plasmid

#69030

Purpose

Reporter Plasmid - deGFP driven by a qacR responsive promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

deGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

qacA

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-NSP4SA11-hPEST

Plasmid

#220820

Purpose

Express the Rotavirus SA11 NSP4 fused with the PEST degradation sequence from mouse ornithine decarboxylase

Depositor

Alex Borodavka

Insert

Rotavirus SA11 NSP4-hPEST

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-LEU-IpgD

Plasmid

#183672

Purpose

Inducible Shigella flexneri IpgD for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRV∆G-NPEST-4Cre

Plasmid

#174380

Purpose

Rabies vector for expressing Cre recombinase and N gene with PEST domain

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

Cre recombinase

Use

Rabies

Tags

Expression

Mutation

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRV∆G-N*PEST-4Cre

Plasmid

#174381

Purpose

Rabies vector for expressing Cre recombinase and N gene with a stop codon before PEST domain

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

Cre recombinase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pESRRG.1.0-gDNA

Plasmid

#132473

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

ESRRG (ESRRG Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pES004 (pTet-qacR 5)

Plasmid

#69033

Purpose

qacR 5 downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

W61Y, E90Q, F102S, M116Q, Y119L, E120Q

Promoter

pTet

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pES003 (pTet-qacR 2)

Plasmid

#69032

Purpose

qacR 2 downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

Promoter

pTet

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS-PesaR-Dlux

Plasmid

#47653

Purpose

PesaR-D promoter in pCS26

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

PesaR-D

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Added an additional esa box upstream of -35 site …

Promoter

PesaR-D

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pLDLR-Luc (aka: pES7)

Plasmid

#14940

Depositor

Axel Nohturfft

Article

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

LDLR promoter (LDLR Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pES001 MBP-GSK3β-HA-His

Plasmid

#154010

Purpose

Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GSK3B (GSK3B Human)

Use

Tags

HA tag, His6, and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pES002 MBP-GSK3β_S9A-HA-His

Plasmid

#196184

Purpose

Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

Insert

GSK3B (GSK3B Human)

Use

Tags

HA, His6, and MBP

Expression

Bacterial

Mutation

changed Serine 9 to Alanine

Promoter

Tac

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-REVERBA-mScarlet-NLS-PEST

Plasmid

#190658

Purpose

Fluorescent reporter for circadian rhythms

Depositor

Mary Teruel

Article

Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

Insert

Reverba-mScarlet (Nr1d1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TetO7-HSP30UTR-nLucPEST-MS2(v4)

Plasmid

#199764

Purpose

Control construct of elongation reporter to quantify elongation of LacZ

Depositor

Brian Zid

Article

Guzikowski et al RNA Biol. 2022;19(1):636-649. doi: 10.10

Insert

NanolucPEST

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHMGCS-Luc (aka: pES8)

Plasmid

#14947

Depositor

Axel Nohturfft

Article

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

HMG CoA synthase promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC165-20XUAS-IVS-R::PEST

Plasmid

#32142

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

R::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPaint-2A-TurboGFP-PEST-PuroR

Plasmid

#80956

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TurboGFP-PEST

Use

Gene tagging

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-URA-GFP-Ubc9ts

Plasmid

#20369

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Mutation

Y68L This mutation renders Ubc9 temperature-sens…

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pESC-LEU-GFP-VHL

Plasmid

#21053

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pJFRC161-20XUAS-IVS-KD::PEST

Plasmid

#32140

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

KD::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge